Bacteriocin: Enterocin K1
Accession: BAC230
Classification
Class: Leaderless
Genetics
Gene: OKA_05812
Producer and target organisms
Producer Organism: Enterococcus faecium EnGen0026 [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Target organismsStaphylococcus aureus (MIC >5500 nM) - Staphylococcus epidermidis (MIC >5500 nM) - Enterococcus faecalis (MIC= 2700- >5500 nM) - Enterococcus faecium (MIC= 10-85 nM) - Enterococcus hirae (MIC= 45 nM) - Lactococcus garvieae (MIC= 340-1370 nM) - Lactobacillus spp. (MIC= 5500- >5500 nM) - Lactococcus lactis 1L1403 (MIC= 170 nM) - Lactococcus lactic (MIC >5500 nM) - Bacillus cereus (MIC= 2750- >5500 nM) - Listeria monocytogenes (MIC >5500 nM)
Description
NULL
Uniprot and PDB links
UniProt Entry: L2P7L3
PDB Entry5L82 resolved by NMR
STRUCTURE AND FUNCTION
PubMed=28515717;DOI=10.3389/fmicb.2017.00774
Ovchinnikov K.V., Kristiansen P.E., Straume D., Jensen M.S., Aleksandrzak-Piekarczyk T., Nes I.F., Diep D.B.
"The Leaderless Bacteriocin Enterocin K1 Is Highly Potent against Enterococcus faecium: A Study on Structure, Target Spectrum and Receptor.", Front Microbiol 2017, 8 ():774.
PubMed=28515717;DOI=10.3389/fmicb.2017.00774
Ovchinnikov K.V., Kristiansen P.E., Straume D., Jensen M.S., Aleksandrzak-Piekarczyk T., Nes I.F., Diep D.B.
"The Leaderless Bacteriocin Enterocin K1 Is Highly Potent against Enterococcus faecium: A Study on Structure, Target Spectrum and Receptor.", Front Microbiol 2017, 8 ():774.
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Protein sequence
| ........10 ........20 ........30 ........40 | | | | MKFKFNPTGT IVKKLTQYEI AWFKNKHGYY PWEIPRC |
Wheel representation
3D Structure
View PDB entry: 5L82
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔37 | 37 | Bacteriocin Enterocin K1.
Feature identifier = |
| HELIX | 8↔24 | 17 |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 1 | 2.70 % |
| C | 1 | 2.70 % |
| D | 0 | 0.00 % |
| E | 2 | 5.41 % |
| F | 3 | 8.11 % |
| G | 2 | 5.41 % |
| H | 1 | 2.70 % |
| I | 3 | 8.11 % |
| K | 6 | 16.22 % |
| L | 1 | 2.70 % |
| M | 1 | 2.70 % |
| N | 2 | 5.41 % |
| P | 3 | 8.11 % |
| Q | 1 | 2.70 % |
| R | 1 | 2.70 % |
| S | 0 | 0.00 % |
| T | 3 | 8.11 % |
| V | 1 | 2.70 % |
| W | 2 | 5.41 % |
| Y | 3 | 8.11 % |
Hydrophobicity
Composition
| Formula | C
H
N
Unknown error type: [8] Undefined offset: 1 O1 |
| Absent amino acids | DS |
| Common amino acids | K |
| Mass (Da) | 4564.88 |
| Net charge | +6 |
| Isoelectric point | 10.24 |
| Basic residues | 8 |
| Acidic residues | 2 |
| Hydrophobic residues | 11 |
| Polar residues | 11 |
| Aliphatic residues | 5 |
| Tiny residues | 3 |
| Boman Index | -48.81 |
| Hydropathy Index | -0.7 |
| Aliphatic Index | 52.7 |
| Instability Index | -0.45 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 15470 M-1 cm-1 |
| Absorbance 280nm | 429.72 |