Bacteriocin: Paenicidin A
Accession: BAC215
Classification
Class: Lantibiotic
Genetics
Gene: paeA
Producer and target organisms
Producer Organism: Paenibacillus polymyxa NRRL B-30509 (Bacillus polymyxa) [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesPaenibacillaceaePaenibacillus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: K7TS19
PDB EntryUnknown
STRUCTURE BY NMR, STRAIN=Paenibacillus polymyxa NRRL B-30509.
PubMed=23167271;DOI=10.1021/ja3089229
Lohans C.T., Huang Z., van Belkum M.J., Giroud M., Sit C.S., Steels E.M., Zheng J., Whittal R.M., McMullen L.M., Vederas J.C.
"Structural Characterization of the Highly Cyclized Lantibiotic Paenicidin A via a Partial Desulfurization/Reduction Strategy.", J Am Chem Soc. (2012).
PubMed=23167271;DOI=10.1021/ja3089229
Lohans C.T., Huang Z., van Belkum M.J., Giroud M., Sit C.S., Steels E.M., Zheng J., Whittal R.M., McMullen L.M., Vederas J.C.
"Structural Characterization of the Highly Cyclized Lantibiotic Paenicidin A via a Partial Desulfurization/Reduction Strategy.", J Am Chem Soc. (2012).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene957 | paeA | PaeA paenicidin A structural gene | 1..177 |
| BACGene958 | paeF | PaeF ABC transporter ATP binding protein | 346..1074 |
| BACGene959 | paeE | PaeE ABC transporter membrane-bound subunit | 1071..1871 |
| BACGene960 | paeG | PaeG ABC transporter membrane-bound subunit | 1884..2651 |
| BACGene961 | paeB | PaeB lantibiotic dehydratase | 2679..5855 |
| BACGene962 | paeT | PaeT lantibiotic ABC transporter | 5852..7729 |
| BACGene963 | paeC | PaeC lantibiotic cyclase | 7731..9113 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | VLSIVACSSG CGSGKTAASC VETCGNRCFT NVGSLC |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 3 | 8.33 % |
| C | 6 | 16.67 % |
| D | 0 | 0.00 % |
| E | 1 | 2.78 % |
| F | 1 | 2.78 % |
| G | 5 | 13.89 % |
| H | 0 | 0.00 % |
| I | 1 | 2.78 % |
| K | 1 | 2.78 % |
| L | 2 | 5.56 % |
| M | 0 | 0.00 % |
| N | 2 | 5.56 % |
| P | 0 | 0.00 % |
| Q | 0 | 0.00 % |
| R | 1 | 2.78 % |
| S | 6 | 16.67 % |
| T | 3 | 8.33 % |
| V | 4 | 11.11 % |
| W | 0 | 0.00 % |
| Y | 0 | 0.00 % |
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | DHMPQWY |
| Common amino acids | SC |
| Mass (Da) | 3376.5 |
| Net charge | +1 |
| Isoelectric point | 7.73 |
| Basic residues | 2 |
| Acidic residues | 1 |
| Hydrophobic residues | 11 |
| Polar residues | 22 |
| Aliphatic residues | 7 |
| Tiny residues | 14 |
| Boman Index | -16.96 |
| Hydropathy Index | 0.675 |
| Aliphatic Index | 73.06 |
| Instability Index | 20.63 (stable) |
| Half Life |
Mammalian : 100 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 375 M-1 cm-1 |
| Absorbance 280nm | 10.71 |