Enterocin W Alfa

Accession: BAC213

Default view

Bacteriocin: Enterocin W alfa

Accession: BAC213

Classification

Class: Class IIb

Genetics

Gene: enwA

Producer and target organisms

Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsBacillus coagulans JCM 2257 (MIC=7.95µM) - Bacillus circulans JCM 2504 (MIC=31.8µM) - Listeria innocua ATCC 33090 (MIC=7.95µM) - Pediococcus pentosaceus JCM 5885 (MIC=31.6µM) - Enterococcus faecalis JCM 5803 (MIC=15.9µM) - Lactococcus lactis subsp. lactis JCM 7638 (MIC=33.8µM) - Lactococcus lactis subsp. lactis ATCC 19435 (MIC=3.97µM) - Lactobacillus sakeisubsp. sakei JCM 1157 (MIC=7.95µM) -

NOTE: not active against Bacillus subtilis JCM 146 NOTE: not active against Bacillus subtilis JCM 1465 - Kocuria rhizophila NBRC 12708 (>3.2 μM)}

Uniprot and PDB links

UniProt Entry: H3JSS9

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=NKR-4-1.
PubMed=22138996;DOI=10.1128/AEM.06497-11
Sawa N., Wilaipun P., Kinoshita S., Zendo T., Leelawatcharamas V., Nakayama J., Sonomoto K.
"Isolation and Characterization of Enterocin W, a Novel Two-Peptide Lantibiotic Produced by Enterococcus faecalis NKR-4-1.", Appl. Environ. Microbiol. 78:900-903(2012).

Gene structure

Gene id	Name	Description	Location
BACGene953	enwA	enterocin W alfa enw of two-peptide lantibiotic	55..237
BACGene954	enwB	enterocin W beta enw of two-peptide lantibiotic	256..441

Protein sequence

........10 ........20 ........30 ........40
| | | |
KCPWWNLSCH LGNDGKICTY SHECTAGCNA

Wheel representation

Composition

Amino acid	Count	Percent
A	2	6.67 %
C	5	16.67 %
D	1	3.33 %
E	1	3.33 %
F	0	0.00 %
G	3	10.00 %
H	2	6.67 %
I	1	3.33 %
K	2	6.67 %
L	2	6.67 %
M	0	0.00 %
N	3	10.00 %
P	1	3.33 %
Q	0	0.00 %
R	0	0.00 %
S	2	6.67 %
T	2	6.67 %
V	0	0.00 %
W	2	6.67 %
Y	1	3.33 %

Hydrophobicity

Composition

Formula	C₀ H₀ N₀ O₀ S₀
Absent amino acids	FMQRV
Common amino acids	C
Mass (Da)	3
Net charge	+2
Isoelectric point	7.24
Basic residues	4
Acidic residues	2
Hydrophobic residues	7
Polar residues	16
Aliphatic residues	3
Tiny residues	7
Boman Index	-35.69
Hydropathy Index	-0.413
Aliphatic Index	45.67
Instability Index	68.86 (unstable)
Half Life	Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min
Extinction Coefficient	12740 M^-1 cm^-1
Absorbance 280nm	439.31