Epidermicin NI01

Accession: BAC210

Default view

Bacteriocin: Epidermicin NI01

Accession: BAC210

Classification

Class: ~~Unclassified~~

Genetics

Gene: edcA

Producer and target organisms

Producer Organism: Staphylococcus epidermidis 224 [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesStaphylococcus

Target organismsStaphylococcus epidermidis 1 (MIC=1µg/ml) - Staphylococcus epidermidis 2 (MIC=4µg/ml) - Staphylococcus epidermidis 37 (MIC=2µg/ml) - Staphylococcus epidermidis 42 (MIC=2µg/ml) - Staphylococcus epidermidis 44 (MIC=4µg/ml) - Staphylococcus epidermidis 60 (MIC=2µg/ml) - Staphylococcus epidermidis 93 (MIC=2µg/ml) - Staphylococcus epidermidis 156 (MIC=0.0625µg/ml) - Staphylococcus epidermidis RP62A (MIC=2-4µg/ml) - Staphylococcus saprophyticus (MIC=0.5-1µg/ml) - Staphylococcus hominis (MIC=1µg/ml) - Staphylococcus warneri (MIC=1µg/ml) - Enterococcus faecalis (MIC=1µg/ml) - VRE 1 (MIC=1µg/ml) - VRE 2 (MIC=1µg/ml) - VRE 3 (MIC=1µg/ml) - VRE 4 (MIC=1-2µg/ml) - VRE 5 (MIC=0.5-1µg/ml) - VRE 6 (MIC=0.5µg/ml) - MRSA s37 (EMRSA 15) (MIC=1-2µg/ml) - MRSA s41 (EMRSA 15) (MIC=1-2µg/ml) - MRSA s71 (EMRSA 15) (MIC=2µg/ml) - Staphylococcus aureus 1195 (MIC=2µg/ml)

Description

Activity: Epidermicin does not cause hemolysis or cell toxicity at 100x MIC.
Physicochemical properties: Stable at pH range from 2-10.

Uniprot and PDB links

UniProt Entry: H9BG66

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

PROTEIN SEQUENCE, FUNCTION AND BIOPHYSICOCHEMICAL PROPERTIES
PubMed=22155816;DOI=10.1128/AAC.05397-11
Sandiford S., Upton M.
"Identification, characterisation and recombinant expression of epidermicin NI01, a novel unmodified bacteriocin produced by Staphylococcus epidermidis displaying potent activity against staphylococci ", Antimicrob. Agents Chemother. AAC.05397-11 (2011).

Gene structure

Gene id	Name	Description	Location
BACGene947		epidermicin locus structural protein ecdA	1..156

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60
| | | | | |
MAAFMKLIQF LATKGQKYVS LAWKHKGTIL KWINAGQSFE WIYKQIKKLW A

Wheel representation

Composition

Amino acid	Count	Percent
A	6	11.76 %
C	0	0.00 %
D	0	0.00 %
E	1	1.96 %
F	3	5.88 %
G	3	5.88 %
H	1	1.96 %
I	5	9.80 %
K	9	17.65 %
L	5	9.80 %
M	2	3.92 %
N	1	1.96 %
P	0	0.00 %
Q	4	7.84 %
R	0	0.00 %
S	2	3.92 %
T	2	3.92 %
V	1	1.96 %
W	4	7.84 %
Y	2	3.92 %

Hydrophobicity

Composition

Formula	C₀ H₀ N₀ O₀ S₀
Absent amino acids	CDPR
Common amino acids	K
Mass (Da)	6045.7
Net charge	+9
Isoelectric point	10.95
Basic residues	10
Acidic residues	1
Hydrophobic residues	24
Polar residues	10
Aliphatic residues	11
Tiny residues	11
Boman Index	-12.56
Hydropathy Index	-0.02
Aliphatic Index	93.92
Instability Index	19.52 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	24980 M^-1 cm^-1
Absorbance 280nm	499.6