Bacteriocin: Sakacin G
Accession: BAC202
Classification
Class: Class IIa
Genetics
Gene: skgA2
Producer and target organisms
Producer Organism: Lactobacillus sakei [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q8KWU5
PDB EntryUnknown
NUCLEOTIDE SEQUENCE.
MEDLINE=22338308;PubMed=12450870;DOI=10.1128/AEM.68.12.6416-6420.2002
Simon L., Fremaux C., Cenatiempo Y., Berjeaud J.M.
"Sakacin g, a new type of antilisterial bacteriocin.", Appl. Environ. Microbiol. 68:6416-6420(2002).
MEDLINE=22338308;PubMed=12450870;DOI=10.1128/AEM.68.12.6416-6420.2002
Simon L., Fremaux C., Cenatiempo Y., Berjeaud J.M.
"Sakacin g, a new type of antilisterial bacteriocin.", Appl. Environ. Microbiol. 68:6416-6420(2002).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene913 | skgC | SkgC | complement(36..569) |
| BACGene914 | skgI | sakacin G immunity protein SkgI | complement(583..939) |
| BACGene915 | skgA2 | prebacteriocin SkgA2 | complement(1009..1176) |
| BACGene916 | SkgA1 | prebacteriocin | complement(1281..1448) |
| BACGene917 | SkgD | bacteriocin ABC-transporter | 1636..2947 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | KYYGNGVSCN SHGCSVNWGQ AWTCGVNHLA NGGHGVC |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔37 | 37 | sakacin G. Feature identifier = PRO_5000440409. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 2 | 5.41 % |
| C | 4 | 10.81 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 0 | 0.00 % |
| G | 8 | 21.62 % |
| H | 3 | 8.11 % |
| I | 0 | 0.00 % |
| K | 1 | 2.70 % |
| L | 1 | 2.70 % |
| M | 0 | 0.00 % |
| N | 5 | 13.51 % |
| P | 0 | 0.00 % |
| Q | 1 | 2.70 % |
| R | 0 | 0.00 % |
| S | 3 | 8.11 % |
| T | 1 | 2.70 % |
| V | 4 | 10.81 % |
| W | 2 | 5.41 % |
| Y | 2 | 5.41 % |
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | DEFIMPR |
| Common amino acids | G |
| Mass (Da) | 3 |
| Net charge | +4 |
| Isoelectric point | 7.96 |
| Basic residues | 4 |
| Acidic residues | 0 |
| Hydrophobic residues | 9 |
| Polar residues | 23 |
| Aliphatic residues | 5 |
| Tiny residues | 13 |
| Boman Index | -29.32 |
| Hydropathy Index | -0.297 |
| Aliphatic Index | 47.3 |
| Instability Index | -2.78 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 14230 M-1 cm-1 |
| Absorbance 280nm | 395.28 |