Weissellin A

Accession: BAC200

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Bacteriocin: Weissellin A

Accession: BAC200

Classification

Class: Class IIa

Genetics

Gene: ~~Unidentified~~

Producer and target organisms

Producer Organism: Weissella paramesenteroides DX [Gram-positive]

Taxonomy: BacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeWeissella

Target organismsUnknown error type: [8] Uninitialized string offset: -1
Gram-positive: Bacillus cereus LMG13569 - Clostridium sporogenes NCTC533 - Clostridium thiaminolyticum ATCC15579 - Enterococcus faecalis NCTC8176 - Lactobacillus brevis ATCC8287 - Lactobacillus bulgaricus LMG13551 - Lactobacillus casei ATCC344 - Lactobacillus curvatus ATCC51436 - Lactobacillus jensenii ATCC25258 - Lactobacillus plantarum CECT220 - Lactobacillus sakei CECT906T - Lactococcus lactis LM0230 - Lactococcus lactis ATCC11454 - Lactococcus lactis IL1403 - Lactococcus lactis subsp. cremoris MC1363 - Leuconostoc mesenteroides ATCC19254 - Listeria inocua ATCC BAA-680D - Listeria monocytogenes ATCC19111 - Micrococcus luteus CECT241 - Pediococcus acidilactici ATCC25740 - Pediococcus pentosaceus ATCC 33316 - Pediococcus pentosaceus LMG13560 - Staphylococcus carnosus LMG13564 -

NOTE: No activity against Salmonella enteritidis ATCC13076.

Description

Physicochemical properties:
Weissellin A retained activity after exposure to 121 �C for 60 min or to −20 �C for 6 months, and to pH 2.0�10.0.
It was not sensitive to trypsin, α-chymotrypsin, pepsin and papain, but was inactivated by proteinase K.

Uniprot and PDB links

UniProt Entry: B3A0N4

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY, STRAIN=DX.
PubMed=21511463;DOI=10.1016/j.biortech.2011.03.106
Papagianni M., Papamichael E.M.
"Purification, amino acid sequence and characterization of the class IIa bacteriocin weissellin A, produced by Weissella paramesenteroides DX.", Bioresour. Technol. 102:6730-6734(2011).

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
KNYGNGVYCN KHKCSVDWAT FSANIANNSV AMAGLTGGNA GNK

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔43	43	Bacteriocin weissellin-A. Feature identifier = PRO_0000414627.
DISULFID	9↔14	6	By similarity.
NON_TER	43

Composition

Amino acid	Count	Percent
A	6	13.95 %
C	2	4.65 %
D	1	2.33 %
E	0	0.00 %
F	1	2.33 %
G	6	13.95 %
H	1	2.33 %
I	1	2.33 %
K	4	9.30 %
L	1	2.33 %
M	1	2.33 %
N	8	18.60 %
P	0	0.00 %
Q	0	0.00 %
R	0	0.00 %
S	3	6.98 %
T	2	4.65 %
V	3	6.98 %
W	1	2.33 %
Y	2	4.65 %

Hydrophobicity

Composition

Formula	C₀ H₀ N₀ O₀ S₀
Absent amino acids	EPQR
Common amino acids	N
Mass (Da)	4450
Net charge	+4
Isoelectric point	9.33
Basic residues	5
Acidic residues	1
Hydrophobic residues	13
Polar residues	23
Aliphatic residues	5
Tiny residues	15
Boman Index	-55.64
Hydropathy Index	-0.433
Aliphatic Index	52.33
Instability Index	23 (stable)
Half Life	Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min
Extinction Coefficient	8605 M^-1 cm^-1
Absorbance 280nm	204.88