Bacteriocin: Zoocin A
Accession: BAC198
Classification
Class: Unclassified
Genetics
Gene: zooA
Producer and target organisms
Producer Organism: Streptococcus equi subsp. zooepidemicus [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Target organismsGram-positive: Streptococcus pyogenes (4/4) - Streptococcus dysgalactiae subsp. equisimilis (1/1) -
NOTE:not active against Micrococcus luteus - Streptococcus anginosus - Streptococcus uberis - Lactococcus lactis subsp. lactis
Description
SEQUENCE: zoocin A is translated as a 285-aa precursor protein and released into the environment by proteolytic cleavage of a 23-aa leader sequence to yield the functional protein of 262 aa.
MODE OF ACTION:
Zoocin A had weak β-lactamase activity, bound radioactive penicillin covalently, and its streptococcolytic activity was inhibited by penicillin. Thus, zoocin A is a penicillin-binding protein and presumably is a D-alanyl endopeptidase.
MODE OF ACTION:
Zoocin A had weak β-lactamase activity, bound radioactive penicillin covalently, and its streptococcolytic activity was inhibited by penicillin. Thus, zoocin A is a penicillin-binding protein and presumably is a D-alanyl endopeptidase.
Uniprot and PDB links
UniProt Entry: O54308
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=4881.
MEDLINE=97311418;PubMed=9168135;DOI=10.1016/S0378-1119(96)00859-1
Simmonds R.S., Simpson W.J., Tagg J.R.
"Cloning and sequence analysis of zooA, a Streptococcus zooepidemicus gene encoding a bacteriocin-like inhibitory substance having a domain structure similar to that of lysostaphin.", Gene 189:255-261(1997).
MEDLINE=97311418;PubMed=9168135;DOI=10.1016/S0378-1119(96)00859-1
Simmonds R.S., Simpson W.J., Tagg J.R.
"Cloning and sequence analysis of zooA, a Streptococcus zooepidemicus gene encoding a bacteriocin-like inhibitory substance having a domain structure similar to that of lysostaphin.", Gene 189:255-261(1997).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=4881.
Gargis A.S., O'Rourke A.-L.D., Sloan G.L., Simmonds R.S.
"", Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
Gargis A.S., O'Rourke A.-L.D., Sloan G.L., Simmonds R.S.
"", Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=9g.
PubMed=19357799;DOI=10.1007/s00239-009-9221-x
Gargis A.S., O'Rourke A.L., Sloan G.L., Simmonds R.S.
"Prevalence and acquisition of the genes for zoocin A and zoocin A resistance in Streptococcus equi subsp. zooepidemicus.", J. Mol. Evol. 68:498-505(2009).
PubMed=19357799;DOI=10.1007/s00239-009-9221-x
Gargis A.S., O'Rourke A.L., Sloan G.L., Simmonds R.S.
"Prevalence and acquisition of the genes for zoocin A and zoocin A resistance in Streptococcus equi subsp. zooepidemicus.", J. Mol. Evol. 68:498-505(2009).
FUNCTION, MODE OF ACTION
PubMed=15251198;DOI=10.1016/j.femsle.2004.05.038
Heath L.S., Heath H.E., LeBlanc P.A., Smithberg S.R., Dufour M., Simmonds R.S., Sloan G.L.
"The streptococcolytic enzyme zoocin A is a penicillin-binding protein", FEMS Microbiol Lett. 15;236(2):205-11 (2004).
PubMed=15251198;DOI=10.1016/j.femsle.2004.05.038
Heath L.S., Heath H.E., LeBlanc P.A., Smithberg S.R., Dufour M., Simmonds R.S., Sloan G.L.
"The streptococcolytic enzyme zoocin A is a penicillin-binding protein", FEMS Microbiol Lett. 15;236(2):205-11 (2004).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene888 | rgg | Rgg putative transcriptional regulator | 10662..10940 |
| BACGene889 | tnpA | TnpA putative IS200-like transposase | complement(11160..11624) |
| BACGene890 | zif | zoocin A immunity factor encodes producer cell immunity for the endopeptidase zoocin A | complement(11954..13186) |
| BACGene891 | zooA | zoocin A endopeptidase endopeptidase; bacteriocin-like inhibitory substance; similar to lysostaphin | 13433..14290 |
| BACGene892 | tnpB | TnpB putative transposase | complement(14377..15636) |
| BACGene893 | tnpB | TnpB putative IS204/IS1001/IS1096/IS1165-like transposase | complement(15758..17017) |
| BACGene894 | tnpB | putative topology modulation protein FlaR-like protein | 17334..17708 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | ATYTRPLDTG NITTGFNGYP GHVGVDYAVP VGTPVRAVAN GTVKFAGNGA NHPWMLWMAG NCVLIQHADG MHTGYAHLSK ISVSTDSTVK QGQIIGYTGA TGQVTGPHLH FEMLPANPNW QNGFSGRIDP TGYIANAPVF NGTTPTEPTT PTTNLKIYKV DDLQKINGIW QVRNNILVPT DFTWVDNGIA ADDVIEVTSN GTRTSDQVLQ KGGYFVINPN NVKSVGTPMK GSGGLSWAQV NFTTGGNVWL NTTSKDNLLY GK |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 16 | 6.11 % |
| C | 1 | 0.38 % |
| D | 13 | 4.96 % |
| E | 3 | 1.15 % |
| F | 8 | 3.05 % |
| G | 33 | 12.60 % |
| H | 7 | 2.67 % |
| I | 14 | 5.34 % |
| K | 11 | 4.20 % |
| L | 14 | 5.34 % |
| M | 5 | 1.91 % |
| N | 24 | 9.16 % |
| P | 16 | 6.11 % |
| Q | 10 | 3.82 % |
| R | 5 | 1.91 % |
| S | 11 | 4.20 % |
| T | 31 | 11.83 % |
| V | 24 | 9.16 % |
| W | 7 | 2.67 % |
| Y | 9 | 3.44 % |
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | |
| Common amino acids | G |
| Mass (Da) | 27998.62 |
| Net charge | +7 |
| Isoelectric point | 7.79 |
| Basic residues | 23 |
| Acidic residues | 16 |
| Hydrophobic residues | 83 |
| Polar residues | 109 |
| Aliphatic residues | 52 |
| Tiny residues | 60 |
| Boman Index | -287.27 |
| Hydropathy Index | -0.267 |
| Aliphatic Index | 74.35 |
| Instability Index | 9.56 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 51910 M-1 cm-1 |
| Absorbance 280nm | 198.89 |