Bacteriocin: Enterocin Xalpha
Accession: BAC189
Classification
Class: Class IIb
Genetics
Gene: enxA
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsUnknown error type: [8] Uninitialized string offset: -1
Gram-positive: - Enterococcus faecalis JCM 5803 (MIC=50.8nM) - Enterococcus faecalis OU510 (MIC=102nM) - Enterococcus faecium TUA 1344L (MIC=50.8nM) - Enterococcus hirae ATCC 10541 (MIC=50.8nM) - Lactobacillus plantarum ATCC 14917 (MIC=102nM) - Lactobacillus sakei ssp. sakei JCM 1157 (MIC=50.8nM) - Lactococcus lactis ssp. cremoris ATCC 19257 (MIC=813nM) - Listeria innocua ATCC 33090 (MIC=25.4nM)
Description
entXα and entXβ encode a double-glycine-type prepeptide with an 18-residue leader sequence, representing the typical structure of class II bacteriocin prepeptides.
Uniprot and PDB links
UniProt Entry: D7UP03
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=KU-B5.
PubMed=20418437;DOI=10.1128/AEM.02264-09
Hu C.-B., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.", Appl. Environ. Microbiol. 76:4542-4545(2010).
PubMed=20418437;DOI=10.1128/AEM.02264-09
Hu C.-B., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.", Appl. Environ. Microbiol. 76:4542-4545(2010).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=KU-B5.
Hu C., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"", Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
Hu C., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"", Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene872 | hypothetical protein ORFX1; hypothetical protein | 298..444 | |
| BACGene873 | hypothetical protein ORFX2; hypothetical protein | complement(434..766) | |
| BACGene874 | hypothetical protein ORFX3; hypothetical protein | complement(763..978) | |
| BACGene875 | enxA | enterocin Xalpha prepeptide Alpha subunit of enterocin X (two-peptide bacteriocin) | 1255..1431 |
| BACGene876 | enxB | enterocin Xbeta Beta subunit of enterocin X (two-peptide bacteriocin) | 1450..1617 |
| BACGene877 | enxB | hypothetical protein ORFX4; hypothetical protein | 1732..1953 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | SNDSLWYGVG QFMGKQANCI TNHPVKHMII PGYCLSKILG |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 1 | 2.50 % |
| C | 2 | 5.00 % |
| D | 1 | 2.50 % |
| E | 0 | 0.00 % |
| F | 1 | 2.50 % |
| G | 5 | 12.50 % |
| H | 2 | 5.00 % |
| I | 4 | 10.00 % |
| K | 3 | 7.50 % |
| L | 3 | 7.50 % |
| M | 2 | 5.00 % |
| N | 3 | 7.50 % |
| P | 2 | 5.00 % |
| Q | 2 | 5.00 % |
| R | 0 | 0.00 % |
| S | 3 | 7.50 % |
| T | 1 | 2.50 % |
| V | 2 | 5.00 % |
| W | 1 | 2.50 % |
| Y | 2 | 5.00 % |
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | ER |
| Common amino acids | G |
| Mass (Da) | 4420.1 |
| Net charge | +4 |
| Isoelectric point | 8.77 |
| Basic residues | 5 |
| Acidic residues | 1 |
| Hydrophobic residues | 12 |
| Polar residues | 16 |
| Aliphatic residues | 9 |
| Tiny residues | 9 |
| Boman Index | -17.14 |
| Hydropathy Index | 0.008 |
| Aliphatic Index | 85.25 |
| Instability Index | 21.72 (stable) |
| Half Life |
Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 8605 M-1 cm-1 |
| Absorbance 280nm | 220.64 |