Bacteriocin: Lactococcin 972
Accession: BAC188
Classification
Class: Unclassified
Genetics
Gene: lcn972; Synonyms=lclA
Producer and target organisms
Producer Organism: Lactococcus lactis subsp. lactis (Streptococcus lactis) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeLactococcus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: O86283
PDB Entry2LGN resolved by NMR
NUCLEOTIDE SEQUENCE, STRAIN=IPLA 972; PLASMID=pBL1.
MEDLINE=20055640;PubMed=10589723;
Martinez B., Fernandez M., Rodriguez A., Suarez J.E.
"Synthesis of lactococcin 972, a bacteriocin produced by Lactococcus lactis IPLA 972, depends on the expression of a plasmid-encoded bicistronic operon.", Microbiology 145:3155-3161(1999).
MEDLINE=20055640;PubMed=10589723;
Martinez B., Fernandez M., Rodriguez A., Suarez J.E.
"Synthesis of lactococcin 972, a bacteriocin produced by Lactococcus lactis IPLA 972, depends on the expression of a plasmid-encoded bicistronic operon.", Microbiology 145:3155-3161(1999).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=IPLA 972; PLASMID=pBL1.
Martinez B.
"", Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Martinez B.
"", Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=IPLA 972; PLASMID=pBL1.
MEDLINE=20532761;PubMed=11078650;DOI=10.1006/plas.2000.1482
Sanchez C., Hernandez de Rojas A., Martinez B., Arguelles M.E., Suarez J.E., Rodriguez A., Mayo B.
"Nucleotide sequence and analysis of pBL1, a bacteriocin-producing plasmid from Lactococcus lactis IPLA 972.", Plasmid 44:239-249(2000).
MEDLINE=20532761;PubMed=11078650;DOI=10.1006/plas.2000.1482
Sanchez C., Hernandez de Rojas A., Martinez B., Arguelles M.E., Suarez J.E., Rodriguez A., Mayo B.
"Nucleotide sequence and analysis of pBL1, a bacteriocin-producing plasmid from Lactococcus lactis IPLA 972.", Plasmid 44:239-249(2000).
[Ref. 4]
STRUCTURE BY NMR OF 26-91, PLASMID=pBL1.
Turner D.L., Lamosa P., Martinez B.
"Structure and properties of lactococcin 972 from Lactococcus lactis.", Submitted (JUL-2011) to the PDB data bank.
Turner D.L., Lamosa P., Martinez B.
"Structure and properties of lactococcin 972 from Lactococcus lactis.", Submitted (JUL-2011) to the PDB data bank.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene864 | hypothetical acetyltransferase ORF01 | 714..1217 | |
| BACGene865 | hypothetical protein ORF04 | 2142..3017 | |
| BACGene866 | lclA | lactococcin 972 | 3240..3515 |
| BACGene867 | lclB | hypothetical lactococcin 972 immunity protein | 3571..5517 |
| BACGene868 | lclB | hypothetical ATP binding protein ORF07 | 5519..6142 |
| BACGene869 | lclB | transposase ORF09 | complement(6641..7321) |
| BACGene870 | lclB | orfX protein | complement(7836..8519) |
| BACGene871 | repB | RepB | complement(8516..9673) |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 | | | | | | | EGTWQHGYGV SSAYSNYHHG SKTHSATVVN NNTGRQGKDT QRAGVWAKAT VGRNLTEKAS FYYNFW |
Wheel representation
3D Structure
View PDB entry: 2LGN
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔66 | 66 | lactococcin 972. Feature identifier = PRO_5000064324. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 6 | 9.09 % |
| C | 0 | 0.00 % |
| D | 1 | 1.52 % |
| E | 2 | 3.03 % |
| F | 2 | 3.03 % |
| G | 8 | 12.12 % |
| H | 4 | 6.06 % |
| I | 0 | 0.00 % |
| K | 4 | 6.06 % |
| L | 1 | 1.52 % |
| M | 0 | 0.00 % |
| N | 6 | 9.09 % |
| P | 0 | 0.00 % |
| Q | 3 | 4.55 % |
| R | 3 | 4.55 % |
| S | 6 | 9.09 % |
| T | 7 | 10.61 % |
| V | 5 | 7.58 % |
| W | 3 | 4.55 % |
| Y | 5 | 7.58 % |
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | CIMP |
| Common amino acids | G |
| Mass (Da) | 7 |
| Net charge | +8 |
| Isoelectric point | 10.07 |
| Basic residues | 11 |
| Acidic residues | 3 |
| Hydrophobic residues | 17 |
| Polar residues | 32 |
| Aliphatic residues | 6 |
| Tiny residues | 20 |
| Boman Index | -147.04 |
| Hydropathy Index | -0.982 |
| Aliphatic Index | 36.97 |
| Instability Index | 6.75 (stable) |
| Half Life |
Mammalian : 1 hour Yeast : 30 min E. coli : >10 hour |
| Extinction Coefficient | 23950 M-1 cm-1 |
| Absorbance 280nm | 368.46 |