Closticin 574

Accession: BAC187

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Bacteriocin: Closticin 574

Accession: BAC187
Classification
Class: Unclassified
Genetics
Gene: cloA
Producer and target organisms

Producer Organism: Clostridium tyrobutyricum [Gram-positive]

Taxonomy: BacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Target organismsUnavailable data

Description
closticin 574 is concluded to be initially produced as a preproprotein of 310 amino acid residues. It is subsequently processed to a bacteriocin of 82 amino acids. The protein has an unusually long preproprotein sequence of 228 amino acids.
Uniprot and PDB links

UniProt Entry: Q8GCU9

PDB EntryUnknown

[Ref. 1] | View abstract | Export citation
NUCLEOTIDE SEQUENCE, STRAIN=ADRIAT 932.
MEDLINE=22507685;PubMed=12620847;DOI=10.1128/AEM.69.3.1589-1597.2003
Kemperman R., Kuipers A., Karsens H., Nauta A., Kuipers O., Kok J.
"Identification and characterization of two novel clostridial bacteriocins, circularin A and closticin 574.", Appl. Environ. Microbiol. 69:1589-1597(2003).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=ADRIAT 932.
Kemperman R.A., Kuipers A., Karsens H., Nauta A., Kuipers O.P., Kok J.
"", Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
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Gene structure
genome of Closticin 574

Gene idNameDescriptionLocation
BACGene862cloAclosticin 574 CloA; bacteriocin precursor374..1303
BACGene863cloBCloB1388..1675
cloAcloB
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 PNWTKIGKCA GSIAWAIGSG LFGGAKLIKI KKYIAELGGL QKAAKLLVGA TTWEEKLHAG GYALINLAAE LTGVAGIQAN
 CF
Wheel representation
wheel representation of Closticin 574
Composition
Amino acidCountPercent
A1417.07 %
C22.44 %
D00.00 %
E44.88 %
F22.44 %
G1315.85 %
H11.22 %
I89.76 %
K910.98 %
L1012.20 %
M00.00 %
N33.66 %
P11.22 %
Q22.44 %
R00.00 %
S22.44 %
T44.88 %
V22.44 %
W33.66 %
Y22.44 %
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids DMR
Common amino acids A
Mass (Da) 8
Net charge +6
Isoelectric point 10
Basic residues 10
Acidic residues 4
Hydrophobic residues 39
Polar residues 26
Aliphatic residues 20
Tiny residues 29
Boman Index 19.5
Hydropathy Index 0.389
Aliphatic Index 109.76
Instability Index 3.62 (stable)
Half Life Mammalian : >20 hour
Yeast : >20 hour
E. coli : ?
Extinction Coefficient 19605 M-1 cm-1
Absorbance 280nm 242.04