Dysgalacticin

Accession: BAC186

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Bacteriocin: Dysgalacticin

Accession: BAC186
Classification
Class: Unclassified
Genetics
Gene: dysA
Producer and target organisms

Producer Organism: Streptococcus equisimilis [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsGram positive: Streptococcus pyogenes FF22

Description
Dysgalacticin (Synonym: streptococcin G-2580) is the second plasmid-encoded Sec-dependent bacteriocin. Dysgalacticin is a heat-labile 21.5 kDa anionic protein that kills its target without inducing lysis.

MODE OF ACTION:
Dysgalacticin appears to bind to the glucose and mannose trans-porters of the PTS (ref 3). Dysgalacticin was shown to block the up-take of glucose and the nonmetabolizable analog 2-deoxyglucose;it also perturbs the membrane of the cell, causing the dissipation of membrane potential (ref 3).

MALDI-TOF MS : 21 492.5±19.3 Da
Uniprot and PDB links

UniProt Entry: Q14DY1

PDB EntryUnknown

[Ref. 1] | View abstract | Export citation
NUCLEOTIDE SEQUENCE, STRAIN=W2580.
PubMed=16804174;DOI=10.1099/mic.0.28823-0
Heng N.C., Ragland N.L., Swe P.M., Baird H.J., Inglis M.A., Tagg J.R., Jack R.W.
"Dysgalacticin: a novel, plasmid-encoded antimicrobial protein (bacteriocin) produced by Streptococcus dysgalactiae subsp. equisimilis.", Microbiology 152:1991-2001(2006).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=W2580.
Heng N.C.K.
"", Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
[Ref. 3] | View abstract | Export citation
MODE OF ACTION
PubMed=19213799;DOI=10.1093/jac/dkn552
Swe P.M., Cook G.M., Tagg J.R., Jack R.W.
"Mode of action of dysgalacticin: a large heat-labile bacteriocin.", J Antimicrob Chemother 2009, 63 (4):679-86.
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Gene structure
genome of Dysgalacticin

Gene idNameDescriptionLocation
BACGene858copGCopG plasmid copy repressor protein86..223
BACGene859repBRepB replication initiation protein283..909
BACGene860dysIdysgalacticin immunity factor DysI902..1075
BACGene861dysAdysgalacticin preprotein DysA; contains 28-aa Sec-dependent signal peptidecomplement(1102..1764)
copGrepBdysIdysA
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 NETNNFAETQ KEITTNSEAT LTNEDYTKLT SEVKTIYTNL IQYDQTKNKF YVDEDKTEQY YNYDDESIKG VYLMKDSLND
 ELNNNNSSNY SEIINQKISE IDYVLQGNDI NNLIPSNTRV KRSADFSWIQ RCLEEAWGYA ISLVTLKGII NLFKAGKFEA
 AAAKLASATA GRIAGMAALF AFVATCGATT VS
Composition
Amino acidCountPercent
A199.90 %
C21.04 %
D115.73 %
E157.81 %
F73.65 %
G84.17 %
H00.00 %
I157.81 %
K147.29 %
L147.29 %
M21.04 %
N2010.42 %
P10.52 %
Q73.65 %
R42.08 %
S147.29 %
T189.38 %
V84.17 %
W21.04 %
Y115.73 %
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids H
Common amino acids N
Mass (Da) 21505.82
Net charge -8
Isoelectric point 4.38
Basic residues 18
Acidic residues 26
Hydrophobic residues 65
Polar residues 73
Aliphatic residues 37
Tiny residues 41
Boman Index -352.74
Hydropathy Index -0.447
Aliphatic Index 80.89
Instability Index 24.51 (stable)
Half Life Mammalian : 1.4 hour
Yeast : 3 min
E. coli : >10 hour
Extinction Coefficient 27515 M-1 cm-1
Absorbance 280nm 144.06