Bacteriocin: rhamnosin A
Accession: BAC181
Classification
Class: Unclassified
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Lactobacillus rhamnosus [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsMicrococcus lysodeikticus ATCC 4698
Description
MODE OF ACTION:
It may act as a pore-forming protein, creating a channel in the cell membrane.
CLASSIFICATION:
Rhamnosin A does have a specific biological activity as a single- and not a two-peptide bacteriocin; amino acid sequencing of its amino terminus suggests that it is not a class IIc circular bacteriocin; for these two reasons, Dimitrijević et al. (2009) believe that Rhamnosin A is a class IId bacteriocin of the type recently proposed and described.
MASS:
Molecular mass of the whole protein is 6433.7558 Da as determined by ESI.
It may act as a pore-forming protein, creating a channel in the cell membrane.
CLASSIFICATION:
Rhamnosin A does have a specific biological activity as a single- and not a two-peptide bacteriocin; amino acid sequencing of its amino terminus suggests that it is not a class IIc circular bacteriocin; for these two reasons, Dimitrijević et al. (2009) believe that Rhamnosin A is a class IId bacteriocin of the type recently proposed and described.
MASS:
Molecular mass of the whole protein is 6433.7558 Da as determined by ESI.
Uniprot and PDB links
UniProt Entry: P86526
PDB EntryUnknown
PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY, STRAIN=68
MEDLINE=19796123;PubMed=19796123;DOI=10.1111/j.1365-2672.2009.04539.x
Dimitrijevic R., Stojanovic M., Zivkovic I., Peterson A., Jankov R.M., Dimitrijevic L., Gavrovic-Jankulovic M.
"The identification of a low molecular mass bacteriocin, rhamnosin A, produced by Lactobacillus rhamnosus strain 68.", Journal of Applied Microbiology 107(6):2108-2115.
MEDLINE=19796123;PubMed=19796123;DOI=10.1111/j.1365-2672.2009.04539.x
Dimitrijevic R., Stojanovic M., Zivkovic I., Peterson A., Jankov R.M., Dimitrijevic L., Gavrovic-Jankulovic M.
"The identification of a low molecular mass bacteriocin, rhamnosin A, produced by Lactobacillus rhamnosus strain 68.", Journal of Applied Microbiology 107(6):2108-2115.
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Protein sequence
| ........10 ........20 | | AVPAVRKTNE TLD |
Warning: Fragment
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1 | Bacteriocin rhamnosin A. Feature identifier = PRO_0000394468. | |
| UNSURE | 2 | V or K. | |
| UNSURE | 3 | P or T. | |
| NON_TER | 13 |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 2 | 15.38 % |
| C | 0 | 0.00 % |
| D | 1 | 7.69 % |
| E | 1 | 7.69 % |
| F | 0 | 0.00 % |
| G | 0 | 0.00 % |
| H | 0 | 0.00 % |
| I | 0 | 0.00 % |
| K | 1 | 7.69 % |
| L | 1 | 7.69 % |
| M | 0 | 0.00 % |
| N | 1 | 7.69 % |
| P | 1 | 7.69 % |
| Q | 0 | 0.00 % |
| R | 1 | 7.69 % |
| S | 0 | 0.00 % |
| T | 2 | 15.38 % |
| V | 2 | 15.38 % |
| W | 0 | 0.00 % |
| Y | 0 | 0.00 % |
Hydrophobicity
Composition
| Formula | C60
H104
N18
O21
|
| Absent amino acids | CFGHIMQSWY |
| Common amino acids | ATV |
| Mass (Da) | 1431.8 |
| Net charge | 0 |
| Isoelectric point | 6.5 |
| Basic residues | 2 |
| Acidic residues | 2 |
| Hydrophobic residues | 5 |
| Polar residues | 3 |
| Aliphatic residues | 3 |
| Tiny residues | 2 |
| Boman Index | -31.16 |
| Hydropathy Index | -0.47 |
| Aliphatic Index | 90 |
| Instability Index | 27.3 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 0 M-1 cm-1 |
| Absorbance 280nm | 0 |