Bacteriocin: Microcin-24
Accession: BAC176
Classification
Class: Unclassified
Genetics
Gene: mtfS
Producer and target organisms
Producer Organism: Escherichia coli [Gram-negative]
Taxonomy: Bacteria
Proteobacteria
Gammaproteobacteria
Enterobacteriales
Enterobacteriaceae
Escherichia
Target organismsUnavailable data
Description
Channel-forming bacteriocin
Uniprot and PDB links
UniProt Entry: Q46971
PDB EntryUnknown
[Ref. 1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].STRAIN=2424;
O'Brien G.J., Mahanty H.K.
"Complete nucleotide sequence of microcin 24 genetic region andanalysis of a new ABC transporter.", Submitted (JAN-1996) to the EMBL/GenBank/DDBJ database
O'Brien G.J., Mahanty H.K.
"Complete nucleotide sequence of microcin 24 genetic region andanalysis of a new ABC transporter.", Submitted (JAN-1996) to the EMBL/GenBank/DDBJ database
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Gene structure
| Gene id | Name | Description | Location |
| BACGene815 | mdbA | microcin DNA binding protein | 94..609 |
| BACGene816 | mtfI | microcin 24 immunity protein immunity to microcin 24 | 854..1135 |
| BACGene817 | mtfS | microcin 24 precursor toxin mcc24 | 1126..1398 |
| BACGene818 | mtfA | MtfA microcin 24 transport | 1873..3117 |
| BACGene819 | mtfB | MtfB mirocin 24 transport (ABC transporter) | 3107..5230 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | AGDPLADPNS QIVRQIMSNA AWGPPLVPER FRGMAVGAAG GVTQTVLQGA AAHMPVNVPI PKVPMGPSWN GSKG |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔74 | 74 | Microcin-24. Feature identifier = PRO_0000005678. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 10 | 13.51 % |
| C | 0 | 0.00 % |
| D | 2 | 2.70 % |
| E | 1 | 1.35 % |
| F | 1 | 1.35 % |
| G | 10 | 13.51 % |
| H | 1 | 1.35 % |
| I | 3 | 4.05 % |
| K | 2 | 2.70 % |
| L | 3 | 4.05 % |
| M | 4 | 5.41 % |
| N | 4 | 5.41 % |
| P | 10 | 13.51 % |
| Q | 4 | 5.41 % |
| R | 3 | 4.05 % |
| S | 4 | 5.41 % |
| T | 2 | 2.70 % |
| V | 8 | 10.81 % |
| W | 2 | 2.70 % |
| Y | 0 | 0.00 % |
Hydrophobicity
Composition
| Formula | C332
H529
N97
O95
S4 |
| Absent amino acids | CY |
| Common amino acids | AGP |
| Mass (Da) | 7546.94 |
| Net charge | +3 |
| Isoelectric point | 10.76 |
| Basic residues | 6 |
| Acidic residues | 3 |
| Hydrophobic residues | 27 |
| Polar residues | 20 |
| Aliphatic residues | 14 |
| Tiny residues | 24 |
| Boman Index | -45.85 |
| Hydropathy Index | -0.03 |
| Aliphatic Index | 76.49 |
| Instability Index | 36.99 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 11000 M-1 cm-1 |
| Absorbance 280nm | 150.68 |
