Bacteriocin: Enterocin HF
Accession: BAC175
Classification
Class: class IIa
Genetics
Gene: EntHF
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: Bacteria
Firmicutes
Bacilli
Lactobacillales
Enterococcaceae
Enterococcus
Target organismsUnavailable data
Uniprot and PDB links
PDB Entry2N4K resolved by NMR
[Ref. 1]
PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.STRAIN=HS, and TA29;
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocinproduced by E. faecium HS and E. faecium TA29 isolated from humans andfish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtK
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocinproduced by E. faecium HS and E. faecium TA29 isolated from humans andfish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtK
[Ref. 2]
PROTEIN SEQUENCE OF 16-58, FUNCTION, AND MASS SPECTROMETRY, STRAIN=HS, and TA29.
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocin produced by E. faecium HS and E. faecium TA29 isolated from humans and fish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtKB.
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocin produced by E. faecium HS and E. faecium TA29 isolated from humans and fish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtKB.
SOLUTION STRUCTURE
PubMed=26585399;DOI=10.1021/acs.jafc.5b03882
Arbulu S., Lohans C.T., Van Belkum M.J., Cintas L.M., Herranz C., Vederas J.C., Hernández P.E.
"Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31.", J Agric Food Chem 2015, ():.
PubMed=26585399;DOI=10.1021/acs.jafc.5b03882
Arbulu S., Lohans C.T., Van Belkum M.J., Cintas L.M., Herranz C., Vederas J.C., Hernández P.E.
"Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31.", J Agric Food Chem 2015, ():.
Loading...
Gene structure
| Gene id | Name | Description | Location |
| BACGene812 | entHF | enterocin HF pediocin-like bacteriocin; EntHF | 198..374 |
| BACGene813 | entHF | putative EntHF ABC-transporter ORF2 | 518..2536 |
| BACGene814 | entHF | putative EntHF immunity protein ORF3 | 2596..2892 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | KYYGNGVSCN KKGCSVDWGK AIGIIGNNAA ANLTTGGKAG WKG |
Wheel representation
3D Structure
View PDB entry: 2N4K
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔43 | 43 | Enterocin HF.
Feature identifier = |
| DISULFID | 9↔14 | 6 | |
| STRAND | 2 | ||
| STRAND | 6↔9 | 4 | |
| STRAND | 13↔16 | 4 | |
| HELIX | 19↔30 | 12 |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 5 | 11.63 % |
| C | 2 | 4.65 % |
| D | 1 | 2.33 % |
| E | 0 | 0.00 % |
| F | 0 | 0.00 % |
| G | 10 | 23.26 % |
| H | 0 | 0.00 % |
| I | 3 | 6.98 % |
| K | 6 | 13.95 % |
| L | 1 | 2.33 % |
| M | 0 | 0.00 % |
| N | 5 | 11.63 % |
| P | 0 | 0.00 % |
| Q | 0 | 0.00 % |
| R | 0 | 0.00 % |
| S | 2 | 4.65 % |
| T | 2 | 4.65 % |
| V | 2 | 4.65 % |
| W | 2 | 4.65 % |
| Y | 2 | 4.65 % |
Hydrophobicity
Composition
| Formula | C188
H295
N55
O57
S3 |
| Absent amino acids | EFHMPQR |
| Common amino acids | G |
| Mass (Da) | 4328.1 |
| Net charge | +5 |
| Isoelectric point | 10.12 |
| Basic residues | 6 |
| Acidic residues | 1 |
| Hydrophobic residues | 13 |
| Polar residues | 23 |
| Aliphatic residues | 6 |
| Tiny residues | 17 |
| Boman Index | -34.01 |
| Hydropathy Index | -0.374 |
| Aliphatic Index | 61.4 |
| Instability Index | 4.29 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 335.83 |
