Bacteriocin: Propionicin-F
Accession: BAC166
Producer Organism: Propionibacterium freudenreichii subsp. freudenreichii LMGT 2946 [Gram-positive]
Taxonomy: Bacteria
ActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium
Target organismsPropionibacterium freudenreichii ssp. freudenreichii ATCC 6207 - Propionibacterium freudenreichii ssp. shermanii ATCC 9614 - Propionibacterium freudenreichii ATCC 9616 - Propionibacterium freudenreichii IFO12426 - Propionibacterium freudenreichii LMGT 2842 - Propionibacterium freudenreichii LMGT 3001 - Propionibacterium freudenreichii ISU-P24 - Propionibacterium freudenreichii ISU-P59 - Propionibacterium freudenreichii ISU-P83 - Propionibacterium freudenreichii ISU-P98 - Propionibacterium freudenreichii ISU-P104 -
note: not active against Propionibacterium acidipropionici ATCC 4965 - Propionibacterium acidipropionici ATCC 4875 - Propionibacterium freudenreichii LMGT 2946 - Propionibacterium freudenreichii LMGT 2956 - Propionibacterium jensenii ATCC 4868 - Propionibacterium jensenii ATCC 14072 - Propionibacterium thoenii ATCC 4872 - Propionibacterium thoenii ATCC 4874
UniProt Entry: Q6E3K9
PDB EntryUnknown
DOI=10.1128/AEM.70.12.7303-7310.2004
Brede D.A., Faye T., Johnsborg O., Oedegaard I., Nes I.F., Holo H
"Molecular and genetic characterization of propionicin F, a bacteriocin from Propionibacterium freudenreichii", Appl. Environ. Microbiol. 70:7303-7310(2004)
| Gene id | Name | Description | Location |
| BACGene766 | putative sugar transport protein orf1 | 22..1431 | |
| BACGene767 | putative transposase orf2 | complement(1711..2349) | |
| BACGene768 | pcfA | propionicin F precursor pro-bacteriocin | 2694..3461 |
| BACGene769 | pcfB | putative radical SAM superfamily protein | 3464..4513 |
| BACGene770 | pcfI | immunity protein | 4582..4965 |
| BACGene771 | pcfC | putative proline peptidase | 5169..6527 |
| BACGene772 | pcfD | putative ABC transporter | complement(7543..9141) |
| ........10 ........20 ........30 ........40 ........50 | | | | | WFYQGMNIAI YANIGGVANI IGYTEAAVAT LLGAVVAVAP VVP |
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔43 | 43 | Propionicin-F. Feature identifier = PRO_0000229754. |
| PROPEP | 44↔154 | 111 | /FTId=PRO_0000229755. |
| Amino acid | Count | Percent |
|---|---|---|
| A | 9 | 20.93 % |
| C | 0 | 0.00 % |
| D | 0 | 0.00 % |
| E | 1 | 2.33 % |
| F | 1 | 2.33 % |
| G | 5 | 11.63 % |
| H | 0 | 0.00 % |
| I | 5 | 11.63 % |
| K | 0 | 0.00 % |
| L | 2 | 4.65 % |
| M | 1 | 2.33 % |
| N | 3 | 6.98 % |
| P | 2 | 4.65 % |
| Q | 1 | 2.33 % |
| R | 0 | 0.00 % |
| S | 0 | 0.00 % |
| T | 2 | 4.65 % |
| V | 7 | 16.28 % |
| W | 1 | 2.33 % |
| Y | 3 | 6.98 % |
| Formula | C206
H318
N48
O55
S1 |
| Absent amino acids | CDHKRS |
| Common amino acids | A |
| Mass (Da) | 4397.9 |
| Net charge | -1 |
| Isoelectric point | 3.85 |
| Basic residues | 0 |
| Acidic residues | 1 |
| Hydrophobic residues | 25 |
| Polar residues | 13 |
| Aliphatic residues | 14 |
| Tiny residues | 14 |
| Boman Index | 53.54 |
| Hydropathy Index | 1.2 |
| Aliphatic Index | 131.63 |
| Instability Index | 68.37 (unstable) |
| Half Life |
Mammalian : 2.8 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 9970 M-1 cm-1 |
| Absorbance 280nm | 237.38 |