Uberolysin

Accession: BAC162

Default view

Bacteriocin: Uberolysin

Accession: BAC162

Classification

Class: ~~Unclassified~~

Genetics

Gene: ublA

Producer and target organisms

Producer Organism: Streptococcus uberis strain 42 [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsEnterococcus faecalis - Enterococcus hirae - Lactococcus lactis - Listeria innocua - Listeria ivanovii - Micrococcus luteus - Staphylococcus aureus - Streptococcus agalactiae - Streptococcus anginosus - Streptococcus dysgalactiae - Streptococcus equisimilis - Streptococcus pyogenes - Streptococcus salivarius

Uniprot and PDB links

UniProt Entry: A5H1G9

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE
PubMed=17464077;DOI=10.1099/mic.0.2006/005967-0
Wirawan R.E., Swanson K.M., Kleffmann T., Jack R.W., Tagg J.R.
"Uberolysin: a novel cyclic bacteriocin produced by Streptococcus uberis.", Microbiology, 153(5):1619-30(2007).

Gene structure

Gene id	Name	Description	Location
BACGene750		PurH-like protein ORF3	170..1330
BACGene751		possible orphan response regulator ORF1; ComE-like; may be involved in regulation of ublA	complement(1383..2138)
BACGene752	ublA	UblA circular bacteriocin uberolysin	2438..2668
BACGene753	ublB	UblB putative maturation factor; putative protein involved in uberolysin maturation	2758..4365
BACGene754	ublC	UblC integral membrane protein; possible accessory factor in uberolysin maturation	4346..4891
BACGene755	ublD	UblD ABC-transporter; possible exporter of uberolysin	4876..5532
BACGene756	ublE	UblE ORF2; possible immunity protein	5529..5795

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80
| | | | | | | |
LAGYTGIASG TAKKVVDAID KGAAAFVIIS IISTVISAGA LGAVSASADF IILTVKNYIS RNLKAQAVIW

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
PEPTIDE	1↔70	70	Bacteriocin uberolysin. Feature identifier = PRO_0000326413.
Cross-link	1↔70	70	Cyclopeptide (Leu-Trp).

Composition

Amino acid	Count	Percent
A	14	20.00 %
C	0	0.00 %
D	3	4.29 %
E	0	0.00 %
F	2	2.86 %
G	6	8.57 %
H	0	0.00 %
I	11	15.71 %
K	5	7.14 %
L	4	5.71 %
M	0	0.00 %
N	2	2.86 %
P	0	0.00 %
Q	1	1.43 %
R	1	1.43 %
S	7	10.00 %
T	4	5.71 %
V	7	10.00 %
W	1	1.43 %
Y	2	2.86 %

Hydrophobicity

Composition

Formula	C₃₂₄ H₅₃₄ N₈₂ O₉₃
Absent amino acids	CEHMP
Common amino acids	A
Mass (Da)	7085.54
Net charge	+3
Isoelectric point	10.17
Basic residues	6
Acidic residues	3
Hydrophobic residues	39
Polar residues	21
Aliphatic residues	22
Tiny residues	27
Boman Index	19.34
Hydropathy Index	0.94
Aliphatic Index	132.57
Instability Index	-2.28 (stable)
Half Life	Mammalian : 5.5 hour Yeast : 3 min E. coli : 2 min
Extinction Coefficient	8480 M^-1 cm^-1
Absorbance 280nm	122.9