Columbicin A

Accession: BAC157

Default view

Bacteriocin: Columbicin A

Accession: BAC157

Classification

Class: Lantibiotic

Genetics

Gene: colA

Producer and target organisms

Producer Organism: Enterococcus columbae [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organisms~~Unavailable data~~

Uniprot and PDB links

UniProt Entry: A0FL76

PDB Entry~~Unknown~~

[Ref. 1]

Martin M., Brede D.A., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
""Amino acid and nucleotide sequence of columbicin A, a novel lantibiotic variant produced by Enterococcus columbae PLCH2, isolated from wood pigeons (Columba palumbus)."",

Gene structure

Gene id	Name	Description	Location
BACGene728	colA	columbicin A class I lantibiotic variant; antimicrobial peptide	146..322
BACGene729	colA	hypothetical protein orfB	404..536

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60
| | | | | |
MMNATENQIF VETVSDQELE MLIGGAGRGW IKTLTKDCPN VISSICAGTI ITACKNCA

Wheel representation

Composition

Amino acid	Count	Percent
A	5	8.62 %
C	4	6.90 %
D	2	3.45 %
E	4	6.90 %
F	1	1.72 %
G	5	8.62 %
H	0	0.00 %
I	7	12.07 %
K	3	5.17 %
L	3	5.17 %
M	3	5.17 %
N	4	6.90 %
P	1	1.72 %
Q	2	3.45 %
R	1	1.72 %
S	3	5.17 %
T	6	10.34 %
V	3	5.17 %
W	1	1.72 %
Y	0	0.00 %

Hydrophobicity

Composition

Formula	C₂₆₃ H₄₃₅ N₇₁ O₈₆ S₇
Absent amino acids	HY
Common amino acids	IT
Mass (Da)	6211.2
Net charge	-2
Isoelectric point	4.39
Basic residues	4
Acidic residues	6
Hydrophobic residues	20
Polar residues	22
Aliphatic residues	13
Tiny residues	13
Boman Index	-46.96
Hydropathy Index	0.24
Aliphatic Index	90.86
Instability Index	26.79 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	5500 M^-1 cm^-1
Absorbance 280nm	100.88