Bovicin HJ50

Accession: BAC156

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Bacteriocin: Bovicin HJ50

Accession: BAC156

Classification

Class: Lantibiotic

Genetics

Gene: bovA

Producer and target organisms

Producer Organism: Streptococcus bovis HJ50 [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsBacillus megaterium AS1.941 - Bacillus subtilis AS1.1087 - Lactobacillus curvatus LTH1174 - Leuconostoc mesenteroides AS1.2 - Leuconostoc dextranicum 181 - Micrococcus flavus NCIB8166 - note: not active against Bacillus cereus - Bacillus coagulans AS1.949 - Escherichia coli - Enterococcus faecalis - Lactobacillus acidophilus - Lactobacillus brevis - Lactobacillus buchneri AS1.40 - Lactobacillus plantarum - Listeria monocytogenes - Pseudomonas aeruginosa 10105 - Shigella flexneri 51285 - Staphylococcus aureus 26071

Uniprot and PDB links

UniProt Entry: Q83ZN8

PDB Entry2M8V resolved by NMR

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE of bovicin HJ50
PubMed=14702402;
Xiao H., Chen X., Chen M., Tang S., Zhao X., Huan L.
"Bovicin HJ50, a novel lantibiotic produced by Streptococcus bovis HJ50", Microbiology. 150(Pt 1):103-108(2004)

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE of bovicin HJ50
PubMed=19202107;
Liu G., Zhong J., Ni J., Chen M., Xiao H., Huan L.
"Characteristics of the bovicin HJ50 gene cluster in Streptococcus bovis HJ50.", Microbiology. 155(Pt 2):584-593(2009)

Gene structure

Gene id	Name	Description	Location
BACGene713		DNA methylase	1..2728
BACGene714		hypothetical protein orf1	2798..3097
BACGene715		hypothetical protein orf2	3112..3402
BACGene716	bovA	BovA	3541..3717
BACGene717	bovM	BovM putative lantibiotic modification enzyme	3798..6311
BACGene718	bovT	BovT putative lantibiotic transporter	6325..8367
BACGene719	bovF	BovF putative component of the bovicin HJ50 transporter complex responsible forimmunity to bovicin HJ50	8342..9250
BACGene720	bovE	BovE putative component of the bovicin HJ50 transporter complex responsible forimmunity to bovicin HJ50	9234..9956
BACGene721	bovG	BovG putative component of the bovicin HJ50 transporter complex responsible forimmunity to bovicin HJ50	9969..10679
BACGene722	bovK	BovK histidine kinase for bovicin operon expression	10669..12180
BACGene723	bovR	BovR response regulator for bovicin operon expression	12158..12754
BACGene724	bovI	BovI putative lipopeptide for immunity to bovicin HJ50	12823..12981
BACGene725	sdb1	Sdb1 thiol-disulfide isomerase and thioredoxin	12992..13477
BACGene726	sdb1	hypothetical protein orf3	13691..14341
BACGene727	sdb1	hypothetical protein orf4	14381..14995

Protein sequence

........10 ........20 ........30 ........40
| | | |
GADRGWIKTL TKDCPNVISS ICAGTIITAC KNCA

Wheel representation

3D Structure

View PDB entry: 2M8V

Protein sequence annotations

Feature	Position(s)	Length	Description
PEPTIDE	1↔34	34	Lantibiotic Bovicin HJ50. Feature identifier =
Modified residue	9		d-alpha-aminobutyric acid.
Modified residue	11		d-alpha-aminobutyric acid.
Cross-link	22↔30	9	disulfide bond (Cys-Cys).
TURN	11↔12	2
TURN	18↔21	4

Composition

Amino acid	Count	Percent
A	4	11.76 %
C	4	11.76 %
D	2	5.88 %
E	0	0.00 %
F	0	0.00 %
G	3	8.82 %
H	0	0.00 %
I	5	14.71 %
K	3	8.82 %
L	1	2.94 %
M	0	0.00 %
N	2	5.88 %
P	1	2.94 %
Q	0	0.00 %
R	1	2.94 %
S	2	5.88 %
T	4	11.76 %
V	1	2.94 %
W	1	2.94 %
Y	0	0.00 %

Hydrophobicity

Composition

Formula	C₁₄₇ H₂₄₈ N₄₂ O₄₆ S₄
Absent amino acids	EFHMQY
Common amino acids	I
Mass (Da)	3428.3
Net charge	+2
Isoelectric point	8.34
Basic residues	4
Acidic residues	2
Hydrophobic residues	12
Polar residues	15
Aliphatic residues	7
Tiny residues	9
Boman Index	-28.3
Hydropathy Index	0.276
Aliphatic Index	89.12
Instability Index	22.04 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	5750 M^-1 cm^-1
Absorbance 280nm	174.24