Thermophilin 1277

Accession: BAC155

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Bacteriocin: Thermophilin 1277

Accession: BAC155
Classification
Class: Lantibiotic
Genetics
Gene: tepA
Producer and target organisms

Producer Organism: Streptococcus thermophilus [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsStreptomyces thermophilus SBT10137 - Streptomyces thermophilus SBT10814 - Streptomyces thermophilus ATCC19258 - Streptomyces thermophilus SBT10813 - Streptomyces thermophilus SBT10838 - Streptomyces thermophilus SBT10914 - Lactobacillus acidophilus JCM1132 - Lactobacillus delbrueckii subsp. bulgaricus SBT0164 - Lactobacillus helveticus SBT10511 - Lactobacillus helveticus SBT2171 - Lactobacillus helveticus SBT1270 - Lactococcus lactis subsp. lactis ATCC11454 - Microbacterium lacticum SBT3513 - Microbacterium lacticum BT3514 - Enterococcus fecalis BT3515 - Clostridium butylicum SBT3516 - Clostridium sprogenes IFH 493 - Clostridium tyrobutylicum SBT3517 - Bacillus cereus IFO13494 -

Note: not active against Lactobacillus gasseri SBT2055 - Lactobacillus gasseri JCM1131 - Staphylococcus aureus ATCC25923 - Listeria innocua IFH178 - Listeria innocua IFH180 - Listeria monocytogenes IFH181 - Listeria monocytogenes IFH182 - Listeria monocytogenes IFH179

Description
First report on a lantibiotic produced by Strep. thermophilus strain. The DNA sequence of this region is identical to bovicin HJ50 produced by Strep. bovis HJ50, which belongs to the same genus to Strep. thermophilus.
Uniprot and PDB links

UniProt Entry: A7M695

PDB EntryUnknown

[Ref. 1] | View abstract | Export citation
characterization of thermophilin 1277
PubMed=17381740;DOI=10.1111/j.1365-2672.2006.03159.x
Kabuki T., Uenishi H., Watanabe M., Seto Y., Nakajima H.
"Characterization of a bacteriocin, Thermophilin 1277, produced by Streptococcus thermophilus SBT1277.", J. Appl. Microbiol. 102(4):971-980(2007)
[Ref. 2] | View abstract | Export citation
NUCLEOTIDE SEQUENCE of thermophilin 1277
PubMed=19191960;
Kabuki T., Uenishi H., Seto Y., Yoshioka T., Nakajima H.
"A unique lantibiotic, thermophilin 1277, containing a disulfide bridge and two thioether bridges.", J. Appl. Microbiol. 106(3):853-862(2009)
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Gene structure
genome of Thermophilin 1277

Gene idNameDescriptionLocation
BACGene693hypothetical protein1..1050
BACGene694hypothetical protein1120..1419
BACGene695hypothetical protein1432..1722
BACGene696tepAthermophilin 12771861..2037
BACGene697tepMhypothetical bacteriocin modified enzyme2118..4631
BACGene698tepThypothetical bacteriocin transporter4645..6687
BACGene699tepFhypothetical bacteriocin immunity protein6662..7570
BACGene700tepFhypothetical protein7554..8276
BACGene701tepFhypothetical protein8283..8999
BACGene702tepKhypothetical bacteriocin immunity protein8989..10500
BACGene703tepRhypothetical bacteriocin immunity protein10721..11074
BACGene704tepRhypothetical protein11143..11301
BACGene705tepRhypothetical protein11313..11798
BACGene706tepRhypothetical protein12012..12662
BACGene707tepRhypothetical protein12702..13790
BACGene708tepRhypothetical protein13844..14071
BACGene709tepRhypothetical protein14075..14470
BACGene710tepRhypothetical proteincomplement(14831..15130)
BACGene711tepRhypothetical proteincomplement(15117..15485)
BACGene712tepRhypothetical proteincomplement(15482..16402)
tepAtepMtepTtepFtepFtepFtepKtepRtepRtepRtepRtepRtepRtepRtepRtepRtepR
Protein sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 ADRGWIKTLT KDCPNVISSI CAGTIITACK NCA
Wheel representation
wheel representation of Thermophilin 1277
Composition
Amino acidCountPercent
A412.12 %
C412.12 %
D26.06 %
E00.00 %
F00.00 %
G26.06 %
H00.00 %
I515.15 %
K39.09 %
L13.03 %
M00.00 %
N26.06 %
P13.03 %
Q00.00 %
R13.03 %
S26.06 %
T412.12 %
V13.03 %
W13.03 %
Y00.00 %
Hydrophobicity

Composition
Formula C147 H248 N42 O46 S4
Absent amino acids EFHMQY
Common amino acids I
Mass (Da) 3486.66
Net charge +2
Isoelectric point 8.34
Basic residues 4
Acidic residues 2
Hydrophobic residues 12
Polar residues 14
Aliphatic residues 7
Tiny residues 8
Boman Index -29.24
Hydropathy Index 0.3
Aliphatic Index 91.82
Instability Index 24.98 (stable)
Half Life Mammalian : 4.4 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 5500 M-1 cm-1
Absorbance 280nm 179.69