Bacteriocin: Ubericin A
Accession: BAC150
Classification
Class: Class IIa
Genetics
Gene: ubaA
Producer and target organisms
Producer Organism: Streptococcus uberis [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Target organismsListeria monocytogenes - Listeria grayi - Listeria seeligeri - Listeria ivanovii - Enterococcus faecalis - Streptococcus bovis - Enterococcus hirae - Lactococcus lactis -
note: not active against Streptococcus pyogenes - Streptococcus salivarius - Streptococcus dysgalactiae - Streptococcus gordonii - Streptococcus mitis - Streptococcus mutans - Staphylococcus aureus
Description
FUNCTION:
Heat-stable antibiotic with bacteriolytic activity against Listeria spp., Enterococcus hirae, Enterococcus faecalis ATCC 19433, S.bovis 83, S.uberis 42 and L.lactis.
SUBCELLULAR LOCATION: Secreted.
PTM: The disulfide bond is essential for the activity.
MASS SPECTROMETRY: Mass=5270.5; Mass_error=5.3; Method=MALDI;
Range=22-70; Source=PubMed:17933926;
SIMILARITY: Belongs to the bacteriocin class IIA/YGNGV family.
Heat-stable antibiotic with bacteriolytic activity against Listeria spp., Enterococcus hirae, Enterococcus faecalis ATCC 19433, S.bovis 83, S.uberis 42 and L.lactis.
SUBCELLULAR LOCATION: Secreted.
PTM: The disulfide bond is essential for the activity.
MASS SPECTROMETRY: Mass=5270.5; Mass_error=5.3; Method=MALDI;
Range=22-70; Source=PubMed:17933926;
SIMILARITY: Belongs to the bacteriocin class IIA/YGNGV family.
Uniprot and PDB links
UniProt Entry: A9Q0M7
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-61, DISULFIDE BOND, AND MASS SPECTROMETRY.
PubMed=17933926;DOI=10.1128/AEM.01818-07
Heng N.C.K., Burtenshaw G.A., Jack R.W., Tagg J.R.
"Ubericin A, a class IIa bacteriocin produced by Streptococcus uberis.", Appl. Environ. Microbiol. 73:7763-7766 (2007).
PubMed=17933926;DOI=10.1128/AEM.01818-07
Heng N.C.K., Burtenshaw G.A., Jack R.W., Tagg J.R.
"Ubericin A, a class IIa bacteriocin produced by Streptococcus uberis.", Appl. Environ. Microbiol. 73:7763-7766 (2007).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene680 | ubaA | ubericin A precursor peptide | 294..506 |
| BACGene681 | ubaI | ubericin A immunity protein | 506..805 |
| BACGene682 | fpg | formamidopyrimidine-DNA glycosylase | 985..1806 |
| BACGene683 | coaE | dephospho-CoA kinase | 1803..2390 |
| BACGene684 | coaE | predicted membrane protein unknown function; similar to Haso02000823 from Haemophilus somnus strain 2336;ORF1 | 2400..3035 |
| BACGene685 | coaE | hypothetical protein putative ATPase; ORF2 | 3161..3554 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | KTVNYGNGLY CNQKKCWVNW SETATTIVNN SIMNGLTGGN AGWHSGGRA |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔49 | 49 | Bacteriocin ubericin-A. Feature identifier = PRO_5000298104. |
| DISULFID | 11↔16 | 6 |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 3 | 6.12 % |
| C | 2 | 4.08 % |
| D | 0 | 0.00 % |
| E | 1 | 2.04 % |
| F | 0 | 0.00 % |
| G | 8 | 16.33 % |
| H | 1 | 2.04 % |
| I | 2 | 4.08 % |
| K | 3 | 6.12 % |
| L | 2 | 4.08 % |
| M | 1 | 2.04 % |
| N | 8 | 16.33 % |
| P | 0 | 0.00 % |
| Q | 1 | 2.04 % |
| R | 1 | 2.04 % |
| S | 3 | 6.12 % |
| T | 5 | 10.20 % |
| V | 3 | 6.12 % |
| W | 3 | 6.12 % |
| Y | 2 | 4.08 % |
Hydrophobicity
Composition
| Formula | C227
H347
N69
O71
S3 |
| Absent amino acids | DFP |
| Common amino acids | GN |
| Mass (Da) | 5293.67 |
| Net charge | +4 |
| Isoelectric point | 9.35 |
| Basic residues | 5 |
| Acidic residues | 1 |
| Hydrophobic residues | 13 |
| Polar residues | 28 |
| Aliphatic residues | 7 |
| Tiny residues | 14 |
| Boman Index | -68.38 |
| Hydropathy Index | -0.56 |
| Aliphatic Index | 55.71 |
| Instability Index | 10.76 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 19480 M-1 cm-1 |
| Absorbance 280nm | 408.44 |