Enterocin 96

Accession: BAC149

Default view

Bacteriocin: Enterocin 96

Accession: BAC149

Classification

Class: Class II

Genetics

Gene: ~~Unidentified~~

Producer and target organisms

Producer Organism: Enterococcus faecalis [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsGram positive : Enterococcus faecalis - Enterococcus faecium WHE - Enterococcus hirae - Enterococcus pseudoavium - Enterococcus sulfureus - Enterococcus saccharolyticus - Enterococcus columbae - Lactobacillus plantarum - Lactobacillus acidophilus - Lactobacillus sakei subsp. sakei - Lactobacillus paracasei subsp. paracasei - Lactococcus lactis - Lactococcus lactis subsp. cremoris - Leuconostoc mesenteroides - Bacillus subtilis - Bacillus cereus - Listeria innocua - Listeria monocytogenes - Staphylococcus xylosus - Staphylococcus aureus -

Gram negative : Salmonella enterica serovar Typhimurium - Salmonella enterica serovar Infantis - Klebsiella pneumoniae - Serratia liquefaciens - Proteus vulgaris - Enterobacter cloacae - Escherichia coli.

Description

search in protein databases revealed that enterocin 96 was identical to the C-terminal part of a putative uncharacterized protein from Enterococcus faecalis V583 (accession number EF_B0056) identified after complete genome sequencing of this strain. The amino acid sequence of this protein contains an N-terminal extension of 26 amino acids with two C-terminal 1 glycyl residues. The likely presence of such an extension for enterocin 96 was investigated by sequencing its structural gene.

Uniprot and PDB links

UniProt Entry: Q82YI9

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

PubMed=19411428;DOI=10.1128/AEM.02772-08
Izquierdo, Esther, Wagner, Camille, Marchioni, Eric, Aoude-Werner, Dalal, Ennahar, Said.
"Enterocin 96, a novel class II bacteriocin produced by Enterococcus faecalis WHE 96, isolated from Munster cheese", Appl. Environ. Microbiol. 2009 0: AEM.02772-08.

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
MSKRDCNLMK ACCAGQAVTY AIHSLLNRLG GDSSDPAGCN DIVRKYCK

Wheel representation

Composition

Amino acid	Count	Percent
A	5	10.42 %
C	5	10.42 %
D	4	8.33 %
E	0	0.00 %
F	0	0.00 %
G	4	8.33 %
H	1	2.08 %
I	2	4.17 %
K	4	8.33 %
L	4	8.33 %
M	2	4.17 %
N	3	6.25 %
P	1	2.08 %
Q	1	2.08 %
R	3	6.25 %
S	4	8.33 %
T	1	2.08 %
V	2	4.17 %
W	0	0.00 %
Y	2	4.17 %

Hydrophobicity

Composition

Formula	C₂₁₄ H₃₅₅ N₆₇ O₆₈ S₇
Absent amino acids	EFW
Common amino acids	AC
Mass (Da)	5493.92
Net charge	+4
Isoelectric point	8.5
Basic residues	8
Acidic residues	4
Hydrophobic residues	13
Polar residues	19
Aliphatic residues	8
Tiny residues	13
Boman Index	-86.9
Hydropathy Index	-0.25
Aliphatic Index	71.25
Instability Index	53.47 (unstable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	2980 M^-1 cm^-1
Absorbance 280nm	68.72