Hiracin JM79

Accession: BAC142

Default view

Bacteriocin: hiracin JM79

Accession: BAC142

Classification

Class: Class II sec-dependent

Genetics

Gene: ~~Unidentified~~

Producer and target organisms

Producer Organism: Enterococcus hirae DCH5 [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsLactobacillus helveticus - Lactobacillus curvatus - Lactobacillus bulgaricus - Lactobacillus sakei - Pediococcus pentosaceus - Enterococcus faecium - Enterococcus faecalis - Propionibacterium sp - Propionibacterium acidipropionici - Clostridium tyrobutiricum - Listeria monocytogenes - Listeria ivanovii - Listeria seeligeri - Listeria welshimeri - Listeria grayi - Staphylococcus aureus - Staphylococcus aureus

Uniprot and PDB links

UniProt Entry: Q0Z8B6

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=DCH5.
PubMed=17326750;DOI=10.1111/j.1574-6968.2007.00673.x
Sanchez J., Diep D.B., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
"Amino acid and nucleotide sequence, adjacent genes, and heterologousexpression of hiracin JM79, a sec-dependent bacteriocin produced by Enterococcus hirae DCH5, isolated from Mallard ducks (Anasplatyrhynchos).", FEMS Microbiol. Lett. 270:227-236(2007).

Gene structure

Gene id	Name	Description	Location
BACGene629		class II sec-dependent bacteriocin antimicrobial peptide	272..496
BACGene630		putative immunity protein	516..803
BACGene631		putative mobilization protein ORFC	1245..1625
BACGene632		relaxase/mobilization nuclease domain protein ORFD	1607..2521
BACGene633		hypothetical protein ORFE	2761..2838

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
ATYYGNGLYC NKEKCWVDWN QAKGEIGKII VNGWVNHGPW APRR

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔44	44	Bacteriocin hiracin-JM79. Feature identifier = PRO_0000352779.
CONFLICT	2		T -> G (in Ref. 1; AA sequence).

Composition

Amino acid	Count	Percent
A	3	6.82 %
C	2	4.55 %
D	1	2.27 %
E	2	4.55 %
F	0	0.00 %
G	6	13.64 %
H	1	2.27 %
I	3	6.82 %
K	4	9.09 %
L	1	2.27 %
M	0	0.00 %
N	5	11.36 %
P	2	4.55 %
Q	1	2.27 %
R	2	4.55 %
S	0	0.00 %
T	1	2.27 %
V	3	6.82 %
W	4	9.09 %
Y	3	6.82 %

Hydrophobicity

Composition

Formula	C₂₃₂ H₃₄₀ N₆₆ O₆₁ S₂
Absent amino acids	FMS
Common amino acids	G
Mass (Da)	5093.2
Net charge	+4
Isoelectric point	9.25
Basic residues	7
Acidic residues	3
Hydrophobic residues	14
Polar residues	17
Aliphatic residues	7
Tiny residues	9
Boman Index	-66.02
Hydropathy Index	-0.75
Aliphatic Index	62.05
Instability Index	13.47 (stable)
Half Life	Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	26595 M^-1 cm^-1
Absorbance 280nm	618.49