Bacteriocin: helveticin-J
Accession: BAC134
Classification
Class: Unclassified
Genetics
Gene: hlv
Producer and target organisms
Producer Organism: Lactobacillus helveticus [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsUnavailable data
Description
heat-sensitive bacteriocin.
Uniprot and PDB links
UniProt Entry: P22294
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=481.
MEDLINE=91035244;PubMed=2228964;
Joerger M.C., Klaenhammer T.R.
"Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin helveticin J.", J. Bacteriol. 172:6339-6347(1990).
MEDLINE=91035244;PubMed=2228964;
Joerger M.C., Klaenhammer T.R.
"Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin helveticin J.", J. Bacteriol. 172:6339-6347(1990).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene599 | ORF1 | 1..484 | |
| BACGene600 | helveticin | ORF2 | 1638..1952 |
| BACGene601 | helveticin | helveticin | 1983..2984 |
| BACGene602 | helveticin | ORF4 | 3297..3364 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | MKHLNETTNV RILSQFDMDT GYQAVVQKGN VGSKYVYGLQ LRKGATTILR GYRGSKINNP ILELSGQAGG HTQTWEFAGD RKDINGEERA GQWFIGVKPS KIEGSKIIWA KQIARVDLRN QMGPHYSNTD FPRLSYLNRA GSNPFAGNKM THAEAAVSPD YTKFLIATVE NNCIGHFTIY NLDTINEKLD EKGNSEDVNL ETVKYEDSFI IDNLYGDDNN SIVNSIQGYD LDNDGNIYIS SQKAPDFDGS YYAHH |
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔333 | 333 | Bacteriocin helveticin-J. Feature identifier = PRO_0000110580. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 15 | 5.88 % |
| C | 1 | 0.39 % |
| D | 19 | 7.45 % |
| E | 13 | 5.10 % |
| F | 9 | 3.53 % |
| G | 24 | 9.41 % |
| H | 7 | 2.75 % |
| I | 20 | 7.84 % |
| K | 16 | 6.27 % |
| L | 16 | 6.27 % |
| M | 4 | 1.57 % |
| N | 23 | 9.02 % |
| P | 7 | 2.75 % |
| Q | 11 | 4.31 % |
| R | 10 | 3.92 % |
| S | 17 | 6.67 % |
| T | 14 | 5.49 % |
| V | 12 | 4.71 % |
| W | 3 | 1.18 % |
| Y | 14 | 5.49 % |
Hydrophobicity
Composition
| Formula | C1660
H2550
N460
O523
S6 |
| Absent amino acids | |
| Common amino acids | GNISK |
| Mass (Da) | 37535.28 |
| Net charge | +2 |
| Isoelectric point | 6.01 |
| Basic residues | 45 |
| Acidic residues | 43 |
| Hydrophobic residues | 98 |
| Polar residues | 120 |
| Aliphatic residues | 48 |
| Tiny residues | 56 |
| Boman Index | -668.44 |
| Hydropathy Index | -0.66 |
| Aliphatic Index | 75.86 |
| Instability Index | 27.38 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 55810 M-1 cm-1 |
| Absorbance 280nm | 168.48 |