Halocin H4

Accession: BAC125

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Bacteriocin: Halocin H4

Accession: BAC125
Classification
Class: Unclassified
Genetics
Gene: HALH4
Producer and target organisms

Producer Organism: Halobacterium mediterranei (Haloferax mediterranei) [Gram-]

Taxonomy: ArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax

Target organismsUnavailable data

Description
Has antibacterial activity against other haloarchaeons. Interacts with the membrane of the target cells where it causes permeability changes that result in an ionic imbalance leading to cell lysis and death.
Uniprot and PDB links

UniProt Entry: Q48236

PDB EntryUnknown

[Ref. 1] | View abstract | Export citation
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE. STRAIN=ATCC 33500 / R4
MEDLINE=97144544;PubMed=8990311;
Cheung J., Danna K.J., O'Connor E.M., Price L.B., Shand R.F.;
"Isolation, sequence, and expression of the gene encoding halocin H4,a bacteriocin from the halophilic archaeon Haloferax mediterranei R4.", J. Bacteriol. 179:548-551(1997).
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Gene structure
genome of Halocin H4

Gene idNameDescriptionLocation
BACGene584halH4Halocin H4286110..287189
halH4
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 DIAPPGPNGD PKSVQIDDKY TGAEMYGEGD FRVGLGTDLT MYPPVYRESL GNGSGGWEFD FTVCGSTACR FVDSNGDVKE
 DDKAKEMWWQ EINFNDINQD LYSRNDSDWV GSTPADTQPE FDYTDFALAR DGVTLALTAL NPAMGSLALG ATYFLSDMVN
 WIASQHEDDS SLKRKWDYDG LSGPLYADSS TYLLARDEMT SNSYESFTID NIAVAFPEFP VRTKYYVTFT APDDPSTQSI
 STLEEEGIYR VPATE
Protein sequence annotations

FeaturePosition(s)LengthDescription
CHAIN1↔313313
Halocin-H4.
Feature identifier = PRO_0000013169.
CONFLICT125E -> D (in Ref. 1; AAB58316).
Composition
Amino acidCountPercent
A197.45 %
C20.78 %
D2911.37 %
E166.27 %
F124.71 %
G207.84 %
H10.39 %
I93.53 %
K83.14 %
L176.67 %
M62.35 %
N114.31 %
P155.88 %
Q62.35 %
R93.53 %
S228.63 %
T207.84 %
V135.10 %
W62.35 %
Y145.49 %
Hydrophobicity

Composition
Formula C1253 H1855 N315 O419 S8
Absent amino acids -
Common amino acids D
Mass (Da) 28294.8
Net charge -27
Isoelectric point 3.81
Basic residues 18
Acidic residues 45
Hydrophobic residues 76
Polar residues 89
Aliphatic residues 39
Tiny residues 61
Boman Index -479.59
Hydropathy Index -0.53
Aliphatic Index 8.01
Instability Index 35.68 (stable)
Half Life Mammalian : 1.1 hour
Yeast : 3 min
E. coli : >10 hour
Extinction Coefficient 56840 M-1 cm-1
Absorbance 280nm 182.58