Bacteriocin: paenibacillin
Accession: BAC122
Classification
Class: Unclassified
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Paenibacillus polymyxa (Bacillus polymyxa) [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesPaenibacillaceaePaenibacillus
Target organismsBacillus spp - Clostridium sporogenes - Lactobacillus spp - Lactococcus lactis - Leuconostoc mesenteroides - Listeria spp - Pediococcus cerevisiae - Staphylococcus aureus - Streptococcus agalactiae
Description
Physico-chemical properties:
water solubility, thermal resistance, and stability against acid/alkali (pH 2.0 to 9.0) treatment.
water solubility, thermal resistance, and stability against acid/alkali (pH 2.0 to 9.0) treatment.
Uniprot and PDB links
UniProt Entry: P86013
PDB EntryUnknown
PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, POST-TRANSLATIONALMODIFICATIONS, AND MASS SPECTROMETRY.STRAIN=OSY-DF
PubMed=17071789;DOI=10.1128/AEM.02023-06
He Z., Kisla D., Zhang L., Yuan C., Green-Church K.B., Yousef A.E.
"Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin.", Appl. Environ. Microbiol. 73:168-178(2007).
PubMed=17071789;DOI=10.1128/AEM.02023-06
He Z., Kisla D., Zhang L., Yuan C., Green-Church K.B., Yousef A.E.
"Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin.", Appl. Environ. Microbiol. 73:168-178(2007).
SUBCELLULAR LOCATION, MASS SPECTROMETRY, STRUCTURE BY NMR, ANDPOST-TRANSLATIONAL MODIFICATION.STRAIN=OSY-DF
PubMed=18625234;DOI=10.1016/j.febslet.2008.07.008
He Z., Yuan C., Zhang L., Yousef A.E.
"N-terminal acetylation in paenibacillin, a novel lantibiotic.", FEBS Lett. 582:2787-2792(2008).
PubMed=18625234;DOI=10.1016/j.febslet.2008.07.008
He Z., Yuan C., Zhang L., Yousef A.E.
"N-terminal acetylation in paenibacillin, a novel lantibiotic.", FEBS Lett. 582:2787-2792(2008).
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Protein sequence
| ........10 ........20 ........30 ........40 | | | | ASIIKTTIKV SKAVCKTLTC ICTGSCSNCK |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔30 | 30 | Lantibiotic paenibacillin. Feature identifier = PRO_0000352649. |
| Modified residue | 1 | N-acetylalanine. | |
| Modified residue | 2 | 2,3-didehydroalanine (Ser). | |
| Modified residue | 6 | 2,3-didehydrobutyrine. | |
| Modified residue | 7 | 2,3-didehydrobutyrine. | |
| Modified residue | 27 | 2,3-didehydroalanine (Ser). | |
| Cross-link | 11↔15 | 5 | Lanthionine (Ser-Cys). |
| Cross-link | 17↔20 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 19↔22 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 23↔26 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 25↔29 | 5 | Lanthionine (Ser-Cys). |
| UNSURE | 11 | S or C. | |
| UNSURE | 15 | C or S. | |
| UNSURE | 25 | S or C. | |
| UNSURE | 29 | C or S. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 2 | 6.67 % |
| C | 5 | 16.67 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 0 | 0.00 % |
| G | 1 | 3.33 % |
| H | 0 | 0.00 % |
| I | 4 | 13.33 % |
| K | 5 | 16.67 % |
| L | 1 | 3.33 % |
| M | 0 | 0.00 % |
| N | 1 | 3.33 % |
| P | 0 | 0.00 % |
| Q | 0 | 0.00 % |
| R | 0 | 0.00 % |
| S | 4 | 13.33 % |
| T | 5 | 16.67 % |
| V | 2 | 6.67 % |
| W | 0 | 0.00 % |
| Y | 0 | 0.00 % |
Hydrophobicity
Composition
| Formula | C129
H234
N36
O41
S5 |
| Absent amino acids | DEFHMPQRWY |
| Common amino acids | CKT |
| Mass (Da) | 3124.3 |
| Net charge | +5 |
| Isoelectric point | 9.16 |
| Basic residues | 5 |
| Acidic residues | 0 |
| Hydrophobic residues | 9 |
| Polar residues | 16 |
| Aliphatic residues | 7 |
| Tiny residues | 7 |
| Boman Index | -17.2 |
| Hydropathy Index | 0.54 |
| Aliphatic Index | 91 |
| Instability Index | 19.12 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 0 M-1 cm-1 |
| Absorbance 280nm | 8.62 |