Bacteriocin: Colicin-V (Microcin-V)
Accession: BAC120
Classification
Class: Unclassified
Genetics
Gene: cvaC
Producer and target organisms
Producer Organism: Escherichia coli [Gram-negative]
Taxonomy: Bacteria
ProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Target organismsactive against Escherichia coli (also closely related bacteria) - Enterobacteriaceae
Description
Mode of action:
Colicin V kills sensitive cells by disrupting the membrane potential.
Colicins are polypeptide toxins. export system.
Colicin V kills sensitive cells by disrupting the membrane potential.
Colicins are polypeptide toxins. export system.
Uniprot and PDB links
UniProt Entry: P22522
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=91065315;PubMed=2249654;
Gilson L., Mahanty H.K., Kolter R.
"Genetic analysis of an MDR-like export system: the secretion of colicin V.", EMBO J. 9:3875-3884(1990).
MEDLINE=91065315;PubMed=2249654;
Gilson L., Mahanty H.K., Kolter R.
"Genetic analysis of an MDR-like export system: the secretion of colicin V.", EMBO J. 9:3875-3884(1990).
[Ref. 2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=Various strains
Pinou T., Riley M.A.
"", Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
Pinou T., Riley M.A.
"", Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=98416173;PubMed=9743528;DOI=10.1084/jem.188.6.1091
Otto B.R., van Dooren S.J.M., Nuijens J.H., Luirink J., Oudega B.
"Characterization of a hemoglobin protease secreted by the pathogenic Escherichia coli strain EB1.", J. Exp. Med. 188:1091-1103(1998).
MEDLINE=98416173;PubMed=9743528;DOI=10.1084/jem.188.6.1091
Otto B.R., van Dooren S.J.M., Nuijens J.H., Luirink J., Oudega B.
"Characterization of a hemoglobin protease secreted by the pathogenic Escherichia coli strain EB1.", J. Exp. Med. 188:1091-1103(1998).
PROTEIN SEQUENCE OF 16-62, AND DISULFIDE BOND.
MEDLINE=95039899;PubMed=7952189;
Havarstein L.S., Holo H., Nes I.F.
"The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by Gram-positive bacteria.", Microbiology 140:2383-2389(1994).
MEDLINE=95039899;PubMed=7952189;
Havarstein L.S., Holo H., Nes I.F.
"The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by Gram-positive bacteria.", Microbiology 140:2383-2389(1994).
PROTEIN SEQUENCE OF 16-28, AND SUBCELLULAR LOCATION.
PubMed=8204625;
Fath M.J., Zhang L.H., Rush J., Kolter R.
"Purification and characterization of colicin V from Escherichia coli culture supernatants.", Biochemistry 33:6911-6917(1994).
PubMed=8204625;
Fath M.J., Zhang L.H., Rush J., Kolter R.
"Purification and characterization of colicin V from Escherichia coli culture supernatants.", Biochemistry 33:6911-6917(1994).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene570 | is30 | IS30 transposase highly similar to X00792 | complement(1..402) |
| BACGene571 | is911 | IS911 hypothetical insertion element IS911, similar to E. coli K12 hypothetical IS911 protein (U70214) | complement(359..727) |
| BACGene572 | is911 | hypothetical protein orf1 | 823..1245 |
| BACGene573 | hbp | haemoglobin protease highly similar to L27423 | 1368..5501 |
| BACGene574 | hbp | hypothetical protein orf2 highly similar to X63666 | 5610..6059 |
| BACGene575 | hbp | hypothetical protein orf3 similar to AE000077 | complement(6483..7742) |
| BACGene576 | hbp | hypothetical protein orf4 similar to AE000077 | complement(7940..8287) |
| BACGene577 | hbp | hypothetical protein orf5 similar to Z73101 | complement(8923..9723) |
| BACGene578 | hbp | hypothetical protein orf6 similar to Y14081 | complement(9857..10738) |
| BACGene579 | cvi | colicin V immunity protein related sequence X57525 | 10929..11165 |
| BACGene580 | cvaC | colicin V related sequence X57525 | 11143..11454 |
| BACGene581 | cvaB | colicin V exporter protein related sequence X57525 | complement(11624..12606) |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | ASGRDIAMAI GTLSGQFVAG GIGAAAGGVA GGAIYDYAST HKPNPAMSPS GLGGTIKQKP EGIPSEAWNY AAGRLCNWSP NNLSDVCL |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔88 | 88 | Colicin-V. Feature identifier = PRO_0000005676. |
| DISULFID | 76↔87 | 12 |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 14 | 15.91 % |
| C | 2 | 2.27 % |
| D | 3 | 3.41 % |
| E | 2 | 2.27 % |
| F | 1 | 1.14 % |
| G | 15 | 17.05 % |
| H | 1 | 1.14 % |
| I | 6 | 6.82 % |
| K | 3 | 3.41 % |
| L | 5 | 5.68 % |
| M | 2 | 2.27 % |
| N | 5 | 5.68 % |
| P | 6 | 6.82 % |
| Q | 2 | 2.27 % |
| R | 2 | 2.27 % |
| S | 8 | 9.09 % |
| T | 3 | 3.41 % |
| V | 3 | 3.41 % |
| W | 2 | 2.27 % |
| Y | 3 | 3.41 % |
Hydrophobicity
Composition
| Formula | C381
H594
N108
O120
S4 |
| Absent amino acids | |
| Common amino acids | GA |
| Mass (Da) | 8755.32 |
| Net charge | +1 |
| Isoelectric point | 7.25 |
| Basic residues | 6 |
| Acidic residues | 5 |
| Hydrophobic residues | 31 |
| Polar residues | 36 |
| Aliphatic residues | 14 |
| Tiny residues | 37 |
| Boman Index | -49.96 |
| Hydropathy Index | 0 |
| Aliphatic Index | 74.55 |
| Instability Index | 48.5 (unstable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 15595 M-1 cm-1 |
| Absorbance 280nm | 179.25 |