Bacteriocin: Gassericin T (gassericin K7 B)
Accession: BAC117
Classification
Class: Unclassified
Genetics
Gene: gatA
Producer and target organisms
Producer Organism: Lactobacillus gasseri [Gram-positive]
Taxonomy: Bacteria
Firmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q9XDR7
PDB EntryUnknown
NUCLEOTIDE SEQUENCE.
PubMed=14504837;DOI=10.1007/s00253-003-1424-2
Majhenic A.C., Venema K., Allison G.E., Matijasic B.B., Rogelj I., Klaenhammer T.R.
"DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221.", Appl. Microbiol. Biotechnol. 63:705-714(2004).
PubMed=14504837;DOI=10.1007/s00253-003-1424-2
Majhenic A.C., Venema K., Allison G.E., Matijasic B.B., Rogelj I., Klaenhammer T.R.
"DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221.", Appl. Microbiol. Biotechnol. 63:705-714(2004).
NUCLEOTIDE SEQUENCE, STRAIN=SBT2055
MEDLINE=21012332;PubMed=11129595;DOI=10.1271/bbb.64.2201
Kawai Y., Saitoh B., Takahashi O., Kitazawa H., Saito T.
"Primary amino acid and DNA sequences of gassericin T, a lactacin F- family bacteriocin produced by Lactobacillus gasseri SBT2055.", Biosci. Biotechnol. Biochem. 64:2201-2208(2000).
MEDLINE=21012332;PubMed=11129595;DOI=10.1271/bbb.64.2201
Kawai Y., Saitoh B., Takahashi O., Kitazawa H., Saito T.
"Primary amino acid and DNA sequences of gassericin T, a lactacin F- family bacteriocin produced by Lactobacillus gasseri SBT2055.", Biosci. Biotechnol. Biochem. 64:2201-2208(2000).
[Ref. 3]
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Gene structure
| Gene id | Name | Description | Location |
| BACGene535 | gatP | putative pheromone peptide | 156..308 |
| BACGene536 | gatK | putative histidine kinase | 311..1618 |
| BACGene537 | gatR | putative response regulator | 1615..2412 |
| BACGene538 | gatT | putative ABC-transporter | 2425..4584 |
| BACGene539 | gatC | putative accessory protein | 4595..5188 |
| BACGene540 | gatZ | hypothetical protein | 5256..5357 |
| BACGene541 | gatA | bacteriocin | 5490..5717 |
| BACGene542 | gatX | bacteriocin | 5727..5924 |
| BACGene543 | gatI | putative immunity protein | 5940..6278 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 | | | | | | RNNWAANIGG VGGATVAGWA LGNAVCGPAC GFVGAHYVPI AWAGVTAATG GFGKIRK |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 12 | 21.05 % |
| C | 2 | 3.51 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 2 | 3.51 % |
| G | 13 | 22.81 % |
| H | 1 | 1.75 % |
| I | 3 | 5.26 % |
| K | 2 | 3.51 % |
| L | 1 | 1.75 % |
| M | 0 | 0.00 % |
| N | 4 | 7.02 % |
| P | 2 | 3.51 % |
| Q | 0 | 0.00 % |
| R | 2 | 3.51 % |
| S | 0 | 0.00 % |
| T | 3 | 5.26 % |
| V | 6 | 10.53 % |
| W | 3 | 5.26 % |
| Y | 1 | 1.75 % |
Hydrophobicity
Composition
| Formula | C195
H294
N58
O60
S2 |
| Absent amino acids | DEMQS |
| Common amino acids | GA |
| Mass (Da) | 5542.65 |
| Net charge | +5 |
| Isoelectric point | 10.26 |
| Basic residues | 5 |
| Acidic residues | 0 |
| Hydrophobic residues | 27 |
| Polar residues | 23 |
| Aliphatic residues | 10 |
| Tiny residues | 25 |
| Boman Index | 13.36 |
| Hydropathy Index | 0.46 |
| Aliphatic Index | 21.96 |
| Instability Index | 25.91 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 18115 M-1 cm-1 |
| Absorbance 280nm | 323.48 |
