Bacteriocin: Plantaricin 1.25 β
Accession: BAC115
Classification
Class: Unclassified
Genetics
Gene: plnB
Producer and target organisms
Producer Organism: Lactobacillus plantarum [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsLactobacillus plantarum - plantarum sp - plantarum coryniformis
Description
Lactobacillus plantarum TMW1.25 produces two bacteriocins plantaricin 1.25a and plantaricin 1.25b.
Uniprot and PDB links
UniProt Entry: Q9ZEU9
PDB EntryUnknown
PROTEIN SEQUENCE, STRAIN=TMW1.25
PubMed=10389253;DOI=10.1046/j.1365-2672.1999.00792.x
A. Remiger, V. G. H. Eijsink, M. A. Ehrmann, K. Sletten, I. F. Nes, R. F. Vogel.
"Purification and partial amino acid sequence of plantaricin 1.25? and 1.25?, two bacteriocins produced by Lactobacillus plantarum TMW1.25", Journal of Applied Microbiology 1999, 86 (6): 1053?1058.
PubMed=10389253;DOI=10.1046/j.1365-2672.1999.00792.x
A. Remiger, V. G. H. Eijsink, M. A. Ehrmann, K. Sletten, I. F. Nes, R. F. Vogel.
"Purification and partial amino acid sequence of plantaricin 1.25? and 1.25?, two bacteriocins produced by Lactobacillus plantarum TMW1.25", Journal of Applied Microbiology 1999, 86 (6): 1053?1058.
NUCLEOTIDE SEQUENCE, STRAIN=TMW1.25
MEDLINE=20149857;PubMed=10684981;DOI=10.1016/S0167-4781(00)00003-8
Ehrmann M.A., Remiger A., Eijsink V.GH., Vogel R.F.
"A gene cluster encoding plantaricin 1.25 and other bacteriocin-like peptides in Lactobacillus plantarum TMW1.25.", Biochim. Biophys. Acta 1490:355-361(2000).
MEDLINE=20149857;PubMed=10684981;DOI=10.1016/S0167-4781(00)00003-8
Ehrmann M.A., Remiger A., Eijsink V.GH., Vogel R.F.
"A gene cluster encoding plantaricin 1.25 and other bacteriocin-like peptides in Lactobacillus plantarum TMW1.25.", Biochim. Biophys. Acta 1490:355-361(2000).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene532 | plnB | plantaricin 1.25 beta bacteriocin | 1948..2157 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 | | | | | | KKKKKKVACT WGNAATAAAS GAVXGILGGP TGALAGAIWG VSQCASNNLH GMH |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 11 | 20.75 % |
| C | 2 | 3.77 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 0 | 0.00 % |
| G | 9 | 16.98 % |
| H | 2 | 3.77 % |
| I | 2 | 3.77 % |
| K | 6 | 11.32 % |
| L | 3 | 5.66 % |
| M | 1 | 1.89 % |
| N | 3 | 5.66 % |
| P | 1 | 1.89 % |
| Q | 1 | 1.89 % |
| R | 0 | 0.00 % |
| S | 3 | 5.66 % |
| T | 3 | 5.66 % |
| V | 3 | 5.66 % |
| W | 2 | 3.77 % |
| Y | 0 | 0.00 % |
Hydrophobicity
warning: X is not a standard amino acid.
warning: X is not a standard amino acid.
Composition
| Formula | C220
H358
N68
O62
S3 |
| Absent amino acids | DEFRY |
| Common amino acids | A |
| Mass (Da) | 5172.8 |
| Net charge | +8 |
| Isoelectric point | 10.8 |
| Basic residues | 8 |
| Acidic residues | 0 |
| Hydrophobic residues | 21 |
| Polar residues | 20 |
| Aliphatic residues | 8 |
| Tiny residues | 23 |
| Boman Index | -11.33 |
| Hydropathy Index | 0.083 |
| Aliphatic Index | 21.62 |
| Instability Index | 22.94 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 11125 M-1 cm-1 |
| Absorbance 280nm | 213.94 |