Bacteriocin: Acidocin LF221B (Gassericin K7 B)
Accession: BAC112
Classification
Class: Unclassified
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Lactobacillus gasseri [Gram-positive]
Taxonomy: Bacteria
Firmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q7WRX6
PDB EntryUnknown
NUCLEOTIDE SEQUENCE.
PubMed=14504837;DOI=10.1007/s00253-003-1424-2
Majhenic A.C., Venema K., Allison G.E., Matijasic B.B., Rogelj I., Klaenhammer T.R.
"DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221.", Appl. Microbiol. Biotechnol. 63:705-714(2004).
PubMed=14504837;DOI=10.1007/s00253-003-1424-2
Majhenic A.C., Venema K., Allison G.E., Matijasic B.B., Rogelj I., Klaenhammer T.R.
"DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221.", Appl. Microbiol. Biotechnol. 63:705-714(2004).
[Ref. 2]
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Gene structure
| Gene id | Name | Description | Location |
| BACGene523 | putative ATP-dependent transport protein ORFB1 | 1..1467 | |
| BACGene524 | putative gassericin K7 B accessory protein ORFB2 | 1478..2071 | |
| BACGene525 | putative gassericin K7 B complemental factor GasK7B_alpha; ORFB3 | 2373..2600 | |
| BACGene526 | gassericin K7 B GasK7B_beta; ORFB4 | 2610..2807 | |
| BACGene527 | putative gassericin K7 B immunity protein GaiK7B; ORFB5 | 2823..3161 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | NKWGNAVIGA ATGATRGVSW CRGFGPWGMT ACALGGAAIG GYLGYKSN |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 8 | 16.67 % |
| C | 2 | 4.17 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 1 | 2.08 % |
| G | 12 | 25.00 % |
| H | 0 | 0.00 % |
| I | 2 | 4.17 % |
| K | 2 | 4.17 % |
| L | 2 | 4.17 % |
| M | 1 | 2.08 % |
| N | 3 | 6.25 % |
| P | 1 | 2.08 % |
| Q | 0 | 0.00 % |
| R | 2 | 4.17 % |
| S | 2 | 4.17 % |
| T | 3 | 6.25 % |
| V | 2 | 4.17 % |
| W | 3 | 6.25 % |
| Y | 2 | 4.17 % |
Hydrophobicity
Composition
| Formula | C294
H461
N87
O82
S5 |
| Absent amino acids | DQ |
| Common amino acids | G |
| Mass (Da) | 6705.85 |
| Net charge | +6 |
| Isoelectric point | 10.25 |
| Basic residues | 8 |
| Acidic residues | 2 |
| Hydrophobic residues | 23 |
| Polar residues | 28 |
| Aliphatic residues | 6 |
| Tiny residues | 22 |
| Boman Index | -33.57 |
| Hydropathy Index | 0 |
| Aliphatic Index | 69.23 |
| Instability Index | 14.26 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 19605 M-1 cm-1 |
| Absorbance 280nm | 306.33 |
