Microcin H47

Accession: BAC110

Default view

Bacteriocin: Microcin H47

Accession: BAC110

Classification

Class: ~~Unclassified~~

Genetics

Gene: mchB

Producer and target organisms

Producer Organism: Escherichia coli [Gram-negative]

Taxonomy: BacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Target organismsUnknown error type: [8] Uninitialized string offset: -1
Active on bacteria phylogenetically related to the producing strain.

Description

Mode of action:
Bactericidal antibiotic. ABC transporter system.

Uniprot and PDB links

UniProt Entry: P62530 Q9RM53

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=H47
MEDLINE=99402674;PubMed=10471561;
Rodriguez E., Gaggero C., Lavina M.
"The structural gene for microcin H47 encodes a peptide precursor with antibiotic activity.", Antimicrob. Agents Chemother. 43:2176-2182(1999).

[Ref. 2] | View abstract | Export citation

CHARACTERIZATION, STRAIN=H47
MEDLINE=21091907;PubMed=11181394;DOI=10.1128/AAC.45.3.969-972.2001
Azpiroz M.F., Rodriguez E., Lavina M.
"The structure, function, and origin of the microcin H47 ATP-binding cassette exporter indicate its relatedness to that of colicin V.", Antimicrob. Agents Chemother. 45:969-972(2001).

Gene structure

Gene id	Name	Description	Location
BACGene505	mchA	MchA protein participates in microcin H47 synthesis, IroB homologue, glucosyltransferase	1763..2881
BACGene506	mchS1	MchS1 protein IroD homologue, enterobactin and salmochelin esterase	complement(3030..4295)
BACGene507	mchS2	MchS2 protein Microcin I47 precursor	complement(4593..4826)
BACGene508	mchS3	MchS3 protein Microcin I47 immunity protein	complement(4843..5277)
BACGene509	mchS4	MchS4 protein promotes enterobactin production	complement(5432..5677)
BACGene510	mchX	MchX protein possibly involved in a regulatory loop modulating its own expression and that ofmchI and mchB	5901..6020
BACGene511	mchI	MchI protein Microcin H47 immunity protein	6089..6298
BACGene512	mchB	MchB protein Microcin H47 precursor	6315..6542
BACGene513	mchC	MchC protein participates in Microcin H47 synthesis	6813..8363
BACGene514	mchD	MchD protein participates in Microcin H47 synthesis	8389..8841
BACGene515	mchE	MchE protein membrane-fusion protein, second component of Microcin H47 ABC exporter	8994..10268
BACGene516	mchF	MchF protein ABC protein of Microcin H47 exporter	10261..12357
BACGene517	mcmI (truncated)	McmI (Microcin M immunity protein) truncated protein	12393..12539
BACGene518	mcmA (truncated)	McmA (Microcin M precursor) truncated protein	12610..12831
BACGene519	mcmM (truncated)	McmM (Microcin M-related protein) truncated protein	complement(13347..13637)

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60 ........70
| | | | | | |
GGAPATSANA AGAAAIVGAL AGIPGGPLGV VVGAVSAGLT TAIGSTVGSG SASSSAGGGS

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔60	60	Microcin H47. Feature identifier = PRO_0000021661.
HELIX	15↔35	21	Potential.

Composition

Amino acid	Count	Percent
A	15	25.00 %
C	0	0.00 %
D	0	0.00 %
E	0	0.00 %
F	0	0.00 %
G	16	26.67 %
H	0	0.00 %
I	3	5.00 %
K	0	0.00 %
L	3	5.00 %
M	0	0.00 %
N	1	1.67 %
P	3	5.00 %
Q	0	0.00 %
R	0	0.00 %
S	9	15.00 %
T	4	6.67 %
V	6	10.00 %
W	0	0.00 %
Y	0	0.00 %

Hydrophobicity

Composition

Formula	C₂₀₅ H₃₄₅ N₆₁ O₇₅
Absent amino acids	CDEFHKMQRWY
Common amino acids	GA
Mass (Da)	4883.4
Net charge	0
Isoelectric point	6.11
Basic residues	0
Acidic residues	0
Hydrophobic residues	27
Polar residues	30
Aliphatic residues	12
Tiny residues	40
Boman Index	48.43
Hydropathy Index	0.87
Aliphatic Index	93
Instability Index	17.69 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	0 M^-1 cm^-1
Absorbance 280nm	0