Bacteriocin: Enterocin CRL35 (Mundticin KS)
Accession: BAC108
Classification
Class: Class IIa
Genetics
Gene: munA
Producer and target organisms
Producer Organism: Enterococcus mundtii [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsUnavailable data
PROTEIN SEQUENCE
PubMed=8695065;
Far?as ME, Far?as RN, de Ruiz Holgado AP, Sesma F.
"Purification and N-terminal amino acid sequence of Enterocin CRL 35, a 'pediocin-like' bacteriocin produced by Enterococcus faecium CRL 35. Lett Appl Microbiol. 1996, 22(6):417-9.",
PubMed=8695065;
Far?as ME, Far?as RN, de Ruiz Holgado AP, Sesma F.
"Purification and N-terminal amino acid sequence of Enterocin CRL 35, a 'pediocin-like' bacteriocin produced by Enterococcus faecium CRL 35. Lett Appl Microbiol. 1996, 22(6):417-9.",
NUCLEOTIDE SEQUENCE, STRAIN=CRL35
PubMed=15215149;DOI=10.1128/AAC.48.7.2778-2781.2004
Saavedra L., Minahk C., De Ruiz Holgado A.P., Sesma F.
"Enhancement of the Enterocin CRL35 Activity by a Synthetic Peptide Derived from the NH2-Terminal Sequence.", Antimicrob. Agents Chemother. 48:2778-2781(2004).
PubMed=15215149;DOI=10.1128/AAC.48.7.2778-2781.2004
Saavedra L., Minahk C., De Ruiz Holgado A.P., Sesma F.
"Enhancement of the Enterocin CRL35 Activity by a Synthetic Peptide Derived from the NH2-Terminal Sequence.", Antimicrob. Agents Chemother. 48:2778-2781(2004).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene499 | munA | enterocin CRL35 bacteriocin | 312..488 |
| BACGene500 | mun B | enterocin CRL 35 ATP-dependent transporter involved in translocation and processing of enterocin CRL 35 | 630..2654 |
| BACGene501 | munC | enterocin CRL 35 immunity protein immunity protein | 2679..2975 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | KYYGNGVSCN KKGCSVDWGK AIGIIGNNSA ANLATGGAAG WKS |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 6 | 13.95 % |
| C | 2 | 4.65 % |
| D | 1 | 2.33 % |
| E | 0 | 0.00 % |
| F | 0 | 0.00 % |
| G | 9 | 20.93 % |
| H | 0 | 0.00 % |
| I | 3 | 6.98 % |
| K | 5 | 11.63 % |
| L | 1 | 2.33 % |
| M | 0 | 0.00 % |
| N | 5 | 11.63 % |
| P | 0 | 0.00 % |
| Q | 0 | 0.00 % |
| R | 0 | 0.00 % |
| S | 4 | 9.30 % |
| T | 1 | 2.33 % |
| V | 2 | 4.65 % |
| W | 2 | 4.65 % |
| Y | 2 | 4.65 % |
Hydrophobicity
Composition
| Formula | C186
H291
N55
O58
S2 |
| Absent amino acids | EFHMPQR |
| Common amino acids | G |
| Mass (Da) | 4308.55 |
| Net charge | +4 |
| Isoelectric point | 9.81 |
| Basic residues | 5 |
| Acidic residues | 1 |
| Hydrophobic residues | 14 |
| Polar residues | 23 |
| Aliphatic residues | 6 |
| Tiny residues | 19 |
| Boman Index | -31.82 |
| Hydropathy Index | -0.253 |
| Aliphatic Index | 15.04 |
| Instability Index | 6.27 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 335.83 |