Bacteriocin: lactacin-F (lafA)
Accession: BAC105
Classification
Class: class IIB
Genetics
Gene: lafA; Synonyms=laf; OrderedLocusNames=LJ_0769
Producer and target organisms
Producer Organism: Lactobacillus johnsonii [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsEnterococcus faecalis -
other Lactobacilli
Description
Mode of action:
Heat stable bacteriocin. peptides for activity: lafA and lafX. Associated with a 180 kDa bacteriocin complex.
Heat stable bacteriocin. peptides for activity: lafA and lafX. Associated with a 180 kDa bacteriocin complex.
Uniprot and PDB links
UniProt Entry: P24022
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=ATCC 11506 / VPI 11088
MEDLINE=94113718;PubMed=8285694;
Fremaux C., Ahn C., Klaenhammer T.R.
"Molecular analysis of the lactacin F operon.", Appl. Environ. Microbiol. 59:3906-3915(1993).
MEDLINE=94113718;PubMed=8285694;
Fremaux C., Ahn C., Klaenhammer T.R.
"Molecular analysis of the lactacin F operon.", Appl. Environ. Microbiol. 59:3906-3915(1993).
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], STRAIN=NCC 533
PubMed=14983040;DOI=10.1073/pnas.0307327101
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533.", Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
PubMed=14983040;DOI=10.1073/pnas.0307327101
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533.", Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE, STRAIN=ATCC 11506 / VPI 11088
MEDLINE=91154135;PubMed=1900281;
Muriana P.M., Klaenhammer T.R.
"Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp.", J. Bacteriol. 173:1779-1788(1991).
MEDLINE=91154135;PubMed=1900281;
Muriana P.M., Klaenhammer T.R.
"Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp.", J. Bacteriol. 173:1779-1788(1991).
PROTEIN SEQUENCE OF 19-43, STRAIN=ATCC 11506 / VPI 11088
MEDLINE=91241762;PubMed=1903624;
Muriana P.M., Klaenhammer T.R.
"Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088.", Appl. Environ. Microbiol. 57:114-121(1991).
MEDLINE=91241762;PubMed=1903624;
Muriana P.M., Klaenhammer T.R.
"Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088.", Appl. Environ. Microbiol. 57:114-121(1991).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene477 | lactacin F two-component system inducer peptide precursor | 661355..661507 | |
| BACGene478 | lactacin F two-component system histidine kinase | 661510..662817 | |
| BACGene479 | lactacin F two-component system response regulator | 664315..665112 | |
| BACGene480 | lactacin F ABC transporter permease component | 665125..667284 | |
| BACGene481 | lactacin F transporter auxillary protein | 667295..667888 | |
| BACGene482 | bacteriocin lactacin F, subunit LafA precursor | 668015..668242 | |
| BACGene483 | bacteriocin lactacin F, subunit LafX precursor | 668253..668441 | |
| BACGene484 | lactacin F immunity protein | 668528..668902 | |
| BACGene485 | lactacin F two-component system response regulator fragment | 670446..670853 | |
| BACGene486 | lactacin F ABC transporter permease component | 670866..673025 | |
| BACGene487 | lactacin F transporter auxillary protein | 673036..673629 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 | | | | | | RNNWQTNVGG AVGSAMIGAT VGGTICGPAC AVAGAHYLPI LWTAVTAATG GFGKIRK |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔57 | 57 | Bacteriocin lactacin-F subunit LafA. Feature identifier = PRO_0000002754. |
| CONFLICT | 44 | A -> G (in Ref. 1; no nucleotide entryand 2; AAA16636). |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 10 | 17.54 % |
| C | 2 | 3.51 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 1 | 1.75 % |
| G | 11 | 19.30 % |
| H | 1 | 1.75 % |
| I | 4 | 7.02 % |
| K | 2 | 3.51 % |
| L | 2 | 3.51 % |
| M | 1 | 1.75 % |
| N | 3 | 5.26 % |
| P | 2 | 3.51 % |
| Q | 1 | 1.75 % |
| R | 2 | 3.51 % |
| S | 1 | 1.75 % |
| T | 6 | 10.53 % |
| V | 5 | 8.77 % |
| W | 2 | 3.51 % |
| Y | 1 | 1.75 % |
Hydrophobicity
Composition
| Formula | C248
H395
N73
O70
S3 |
| Absent amino acids | DE |
| Common amino acids | GA |
| Mass (Da) | 5634.49 |
| Net charge | +5 |
| Isoelectric point | 10.26 |
| Basic residues | 5 |
| Acidic residues | 0 |
| Hydrophobic residues | 24 |
| Polar residues | 24 |
| Aliphatic residues | 11 |
| Tiny residues | 22 |
| Boman Index | 0.69 |
| Hydropathy Index | 0.43 |
| Aliphatic Index | 84.04 |
| Instability Index | 23.23 (stable) |
| Half Life |
Mammalian : 1 hour Yeast : 2 min E. coli : 2 min |
| Extinction Coefficient | 12615 M-1 cm-1 |
| Absorbance 280nm | 225.27 |