Bacteriocin: lactococcin-A
Accession: BAC102
Classification
Class: Unclassified
Genetics
Gene: lcnA
Producer and target organisms
Producer Organism: Lactococcus lactis subsp (Streptococcus cremoris), cremoris [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeLactococcus
Target organismsLactococci
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=9B4; PLASMID=p9B4-6
MEDLINE=91197113;PubMed=1901707;
van Belkum M.J., Hayema B.J., Jeeninga R.E., Kok J., Venema G.
"Organization and nucleotide sequences of two lactococcal bacteriocin operons.", Appl. Environ. Microbiol. 57:492-498(1991).
MEDLINE=91197113;PubMed=1901707;
van Belkum M.J., Hayema B.J., Jeeninga R.E., Kok J., Venema G.
"Organization and nucleotide sequences of two lactococcal bacteriocin operons.", Appl. Environ. Microbiol. 57:492-498(1991).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 22-75, STRAIN=LMG 2130
MEDLINE=91267955;PubMed=1904860;
Holo H., Nilssen O., Nes I.F.
"Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene.", J. Bacteriol. 173:3879-3887(1991).
MEDLINE=91267955;PubMed=1904860;
Holo H., Nilssen O., Nes I.F.
"Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene.", J. Bacteriol. 173:3879-3887(1991).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene442 | probacteriocin | 239..466 | |
| BACGene443 | bacteriocin immunity | 481..777 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 | | | | | | KLTFIQSTAA GDLYYNTNTH KYVYQQTQNA FGAAANTIVN GWMGGAAGGF GLHH |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔54 | 54 | Bacteriocin lactococcin-A. Feature identifier = PRO_0000002769.
|
| HELIX | 9↔31 | 23 | Potential |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 8 | 14.81 % |
| C | 0 | 0.00 % |
| D | 1 | 1.85 % |
| E | 0 | 0.00 % |
| F | 3 | 5.56 % |
| G | 8 | 14.81 % |
| H | 3 | 5.56 % |
| I | 2 | 3.70 % |
| K | 2 | 3.70 % |
| L | 3 | 5.56 % |
| M | 1 | 1.85 % |
| N | 5 | 9.26 % |
| P | 0 | 0.00 % |
| Q | 4 | 7.41 % |
| R | 0 | 0.00 % |
| S | 1 | 1.85 % |
| T | 6 | 11.11 % |
| V | 2 | 3.70 % |
| W | 1 | 1.85 % |
| Y | 4 | 7.41 % |
Hydrophobicity
Composition
| Formula | C260
H380
N72
O77
S1 |
| Absent amino acids | CEPR |
| Common amino acids | AG |
| Mass (Da) | 5797.31 |
| Net charge | +4 |
| Isoelectric point | 8.97 |
| Basic residues | 5 |
| Acidic residues | 1 |
| Hydrophobic residues | 19 |
| Polar residues | 24 |
| Aliphatic residues | 7 |
| Tiny residues | 17 |
| Boman Index | -40.24 |
| Hydropathy Index | -0.24 |
| Aliphatic Index | 61.67 |
| Instability Index | 22.35 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 11460 M-1 cm-1 |
| Absorbance 280nm | 216.23 |