Enterocin B

Accession: BAC101

Default view

Bacteriocin: Enterocin B

Accession: BAC101

Classification

Class: class IIc, non subgrouped bacteriocins (problematic)

Genetics

Gene: entB

Producer and target organisms

Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsGram_positive: Enterococcus faecalis JCM 5803 (MIC=86.9nM) - Enterococcus faecalis OU510 (MIC=174nM) - Enterococcus faecium TUA 1344L (MIC=43.4nM) - Enterococcus hirae ATCC 10541 (MIC=5.44nM) - Lactobacillus plantarum ATCC 14917 (MIC=86.9nM) - Lactobacillus sakei ssp. sakei JCM 1157 (MIC=5.44nM) - Bacillus coagulans JCM 2257 (MIC=89000nM) - Bacillus subtilis JCM 1465 (MIC=44500nM)

Uniprot and PDB links

UniProt Entry: O34017

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=T136
MEDLINE=97388583;PubMed=9245817;
Casaus P., Nilsen T., Cintas L.M., Nes I.F., Hernandez P.E., Holo H.
"Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A.", Microbiology 143:0-0(0).

[Ref. 2]

NUCLEOTIDE SEQUENCE, STRAIN=T136
Casaus P., Nielsen T., Cintas L.M., Nes I.F., Hernandez P.E., Holo H.
"", Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.

[Ref. 3] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=BFE 900
MEDLINE=99240446;PubMed=10224016;
Franz C.M.A.P., Worobo R.W., Quadri L.E.N., Schillinger U., Holzapfel W.H., Vederas J.C., Stiles M.E.
"Atypical genetic locus associated with constitutive production of enterocin B by Enterococcus faecium BFE 900.", Appl. Environ. Microbiol. 65:2170-2178(1999).

[Ref. 4] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=KU-B5.
PubMed=20418437;DOI=10.1128/AEM.02264-09
Hu C.-B., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.", Appl. Environ. Microbiol. 76:4542-4545(2010).

Gene structure

Gene id	Name	Description	Location
BACGene419	pepC	cysteine aminopeptidase PepC	complement(1..675)
BACGene420	pepC	unknown orf17	354..494
BACGene421	pepC	unknown orf16	859..1134
BACGene422	pepC	unknown orf15	complement(1098..1259)
BACGene423	pepC	unknown orf14	1131..1283
BACGene424	pepC	unknown orf13	1765..2394
BACGene425	pepC	unknown orf12	2414..2596
BACGene426	pepC	unknown orf11	complement(2508..2882)
BACGene427	bglB	phospho-beta-glucosidase BglB	complement(3195..4622)
BACGene428	bglS	beta-glucoside specific transport protein enzyme II-Bgl; BglS	complement(4634..6490)
BACGene429	bglR	transcription antiterminator BglR	complement(6483..7328)
BACGene430	bglR	unknown orf2	complement(7677..7847)
BACGene431	bglR	unknown orf1	7686..7832
BACGene432	bglR	unknown orf3	complement(7822..8154)
BACGene433	bglR	unknown orf4	complement(8151..8366)
BACGene434	entB	enterocin B precursor bacteriocin	8643..8858
BACGene435	eniB	enterocin B immunity protein EniB	complement(8879..9055)
BACGene436	eniB	unknown orf5	complement(9078..9269)
BACGene437	eniB	induction factor-like protein orf6	complement(9269..9388)
BACGene438	eniB	unknown orf7	10416..10637
BACGene439	eniB	unknown orf8	10917..11108
BACGene440	eniB	unknown orf9	complement(11428..11571)
BACGene441	eniB	unknown orf10	complement(11776..11949)

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60
| | | | | |
ENDHRMPNEL NRPNNLSKGG AKCGAAIAGG LFGIPKGPLA WAAGLANVYS KCN

Wheel representation

Composition

Amino acid	Count	Percent
A	8	15.09 %
C	2	3.77 %
D	1	1.89 %
E	2	3.77 %
F	1	1.89 %
G	8	15.09 %
H	1	1.89 %
I	2	3.77 %
K	4	7.55 %
L	5	9.43 %
M	1	1.89 %
N	7	13.21 %
P	4	7.55 %
Q	0	0.00 %
R	2	3.77 %
S	2	3.77 %
T	0	0.00 %
V	1	1.89 %
W	1	1.89 %
Y	1	1.89 %

Hydrophobicity

Composition

Formula	C₂₃₇ H₃₇₇ N₇₃ O₇₀ S₃
Absent amino acids	QT
Common amino acids	AGN
Mass (Da)	5484.14
Net charge	+4
Isoelectric point	9.43
Basic residues	7
Acidic residues	3
Hydrophobic residues	18
Polar residues	20
Aliphatic residues	8
Tiny residues	18
Boman Index	-61.76
Hydropathy Index	-0.38
Aliphatic Index	72.08
Instability Index	13.58 (stable)
Half Life	Mammalian : 1 hour Yeast : 30 min E. coli : >10 hour
Extinction Coefficient	7115 M^-1 cm^-1
Absorbance 280nm	136.83