Carnobacteriocin-A (Piscicolin-61)

Accession: BAC100

Default view

Bacteriocin: carnobacteriocin-A (Piscicolin-61)

Accession: BAC100

Classification

Class: class IIc, non subgrouped bacteriocins (problematic)

Genetics

Gene: cbnBA; Synonyms=psc61

Producer and target organisms

Producer Organism: Carnobacterium piscicola [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesCarnobacteriaceaeCarnobacterium

Target organismsCarnobacterium - Enterococcus - Listeria monocytogenes - Clostridium perfringens

Uniprot and PDB links

UniProt Entry: P38578

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE, STRAIN=LV17A
MEDLINE=94282297;PubMed=8012574;
Worobo R.W., Henkel T., Sailer M., Roy K.L., Vederas J.C., Stiles M.E.
"Characteristics and genetic determinant of a hydrophobic peptide bacteriocin, carnobacteriocin A, produced by Carnobacterium piscicola LV17A.", Microbiology 140:517-526(1994).

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=LV61
MEDLINE=94297487;PubMed=7764997;
Holck A.L., Axelsson L., Schillinger U.
"Purification and cloning of piscicolin 61, a bacteriocin from Carnobacterium piscicola LV61.", Curr. Microbiol. 29:63-68(1994).

Gene structure

genome of carnobacteriocin-A (Piscicolin-61)

Gene id	Name	Description	Location
BACGene418		carnobacteriocin A	334..549

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60
| | | | | |
DQMSDGVNYG KGSSLSKGGA KCGLGIVGGL ATIPSGPLGW LAGAAGVINS CMK

Wheel representation

Protein sequence annotations

Features of carnobacteriocin-A (Piscicolin-61)

Feature	Position(s)	Length	Description
CHAIN	1↔53	53	Bacteriocin carnobacteriocin-A. Feature identifier = PRO_0000002761.
DISULFID	22↔51	30

Composition

Amino acid	Count	Percent
A	5	9.43 %
C	2	3.77 %
D	2	3.77 %
E	0	0.00 %
F	0	0.00 %
G	13	24.53 %
H	0	0.00 %
I	3	5.66 %
K	4	7.55 %
L	5	9.43 %
M	2	3.77 %
N	2	3.77 %
P	2	3.77 %
Q	1	1.89 %
R	0	0.00 %
S	6	11.32 %
T	1	1.89 %
V	3	5.66 %
W	1	1.89 %
Y	1	1.89 %

Hydrophobicity

Composition

Formula	C₂₁₇ H₃₅₇ N₆₁ O₆₉ S₄
Absent amino acids	EFHR
Common amino acids	G
Mass (Da)	5071.75
Net charge	+2
Isoelectric point	8.78
Basic residues	4
Acidic residues	2
Hydrophobic residues	17
Polar residues	25
Aliphatic residues	11
Tiny residues	24
Boman Index	0.77
Hydropathy Index	0.26
Aliphatic Index	84.72
Instability Index	32.02 (stable)
Half Life	Mammalian : 1.1 hour Yeast : 3 min E. coli : >10 hour
Extinction Coefficient	7115 M^-1 cm^-1
Absorbance 280nm	136.83