Bacteriocin: Lactobin-A (Amylovorin-L471)
Accession: BAC095
Classification
Class: class IIB
Genetics
Gene: amyL
Producer and target organisms
Producer Organism: Lactobacillus amylovorus [Gram-positive]
Taxonomy: Bacteria
Firmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsclosely related Lactobacillus sp - Lactobacillus helveticus ATCC 15009 -
Note:No activity towards Listeria sp
Description
Mode of action:
This heat stable bacteriocin. It may act as a pore- forming protein, creating a channel in the cell membrane. one which is lactobin A. RANGE=16-65; NOTE=Ref.2.
This heat stable bacteriocin. It may act as a pore- forming protein, creating a channel in the cell membrane. one which is lactobin A. RANGE=16-65; NOTE=Ref.2.
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=LMG P-13139
PubMed=14672834;DOI=10.1016/S0168-1605(03)00298-8
De Vuyst L., Avonts L., Neysens P., Hoste B., Vancanneyt M., Swings J., Callewaert R.
"The lactobin A and amylovorin L471 encoding genes are identical, and their distribution seems to be restricted to the species Lactobacillus amylovorus that is of interest for cereal fermentations.", Int. J. Food Microbiol. 90:93-106(2004).
PubMed=14672834;DOI=10.1016/S0168-1605(03)00298-8
De Vuyst L., Avonts L., Neysens P., Hoste B., Vancanneyt M., Swings J., Callewaert R.
"The lactobin A and amylovorin L471 encoding genes are identical, and their distribution seems to be restricted to the species Lactobacillus amylovorus that is of interest for cereal fermentations.", Int. J. Food Microbiol. 90:93-106(2004).
PROTEIN SEQUENCE OF 16-65, AND MASS SPECTROMETRY, STRAIN=LMG P-13139
MEDLINE=97133940;PubMed=8979334;
Contreras B.G.L., De Vuyst L., Devreese B., Busanyova K., Raymaeckers J., Bosman F., Sablon E., Vandamme E.J.
"Isolation, purification, and amino acid sequence of lactobin A, one of the two bacteriocins produced by Lactobacillus amylovorus LMG P- 13139.", Appl. Environ. Microbiol. 63:13-20(1997).
MEDLINE=97133940;PubMed=8979334;
Contreras B.G.L., De Vuyst L., Devreese B., Busanyova K., Raymaeckers J., Bosman F., Sablon E., Vandamme E.J.
"Isolation, purification, and amino acid sequence of lactobin A, one of the two bacteriocins produced by Lactobacillus amylovorus LMG P- 13139.", Appl. Environ. Microbiol. 63:13-20(1997).
PROTEIN SEQUENCE OF 16-50, STRAIN=DCE 471
PubMed=10517609;
Callewaert R., Holo H., Devreese B., Van Beeumen J., Nes I., De Vuyst L.
"Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method.", Microbiology 145:2559-2568(1999).
PubMed=10517609;
Callewaert R., Holo H., Devreese B., Van Beeumen J., Nes I., De Vuyst L.
"Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method.", Microbiology 145:2559-2568(1999).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene388 | amyL | lactobin A bacteriocin | 16..213 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 | | | | | | NRWTNAYSAA LGCAVPGVKY GKKLGGVWGA VIGGVGGAAV CGLAGYVRKG |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔50 | 50 | Bacteriocin amylovorin-L. Feature identifier = PRO_0000002758. |
| TRANSMEM | 24↔44 | 21 | Helical; (Potential). |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 8 | 16.00 % |
| C | 2 | 4.00 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 0 | 0.00 % |
| G | 13 | 26.00 % |
| H | 0 | 0.00 % |
| I | 1 | 2.00 % |
| K | 4 | 8.00 % |
| L | 3 | 6.00 % |
| M | 0 | 0.00 % |
| N | 2 | 4.00 % |
| P | 1 | 2.00 % |
| Q | 0 | 0.00 % |
| R | 2 | 4.00 % |
| S | 1 | 2.00 % |
| T | 1 | 2.00 % |
| V | 7 | 14.00 % |
| W | 2 | 4.00 % |
| Y | 3 | 6.00 % |
Hydrophobicity
Composition
| Formula | C220
H348
N64
O58
S2 |
| Absent amino acids | DEFHMQ |
| Common amino acids | G |
| Mass (Da) | 4900.57 |
| Net charge | +6 |
| Isoelectric point | 10.37 |
| Basic residues | 6 |
| Acidic residues | 0 |
| Hydrophobic residues | 21 |
| Polar residues | 22 |
| Aliphatic residues | 11 |
| Tiny residues | 22 |
| Boman Index | 10.17 |
| Hydropathy Index | 0.38 |
| Aliphatic Index | 87.8 |
| Instability Index | 10.27 (stable) |
| Half Life |
Mammalian : 1.4 hour Yeast : 3 min E. coli : >10 hour |
| Extinction Coefficient | 15595 M-1 cm-1 |
| Absorbance 280nm | 318.27 |
