Lactobin-A (Amylovorin-L471)

Accession: BAC095

Bacteriocin: Lactobin-A (Amylovorin-L471)

Accession: BAC095

Classification
Class: class IIB
Genetics
Gene: amyL
Producer and target organisms

Producer Organism: Lactobacillus amylovorus [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesLactobacillaceaeLactobacillus

Target organismsclosely related Lactobacillus sp - Lactobacillus helveticus ATCC 15009 -

Note:No activity towards Listeria sp

Description
Mode of action:
This heat stable bacteriocin. It may act as a pore- forming protein, creating a channel in the cell membrane. one which is lactobin A. RANGE=16-65; NOTE=Ref.2.
Uniprot and PDB links

UniProt Entry: P80696 P81927 Q8GC39

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=LMG P-13139
PubMed=14672834;DOI=10.1016/S0168-1605(03)00298-8
De Vuyst L., Avonts L., Neysens P., Hoste B., Vancanneyt M., Swings J., Callewaert R.
"The lactobin A and amylovorin L471 encoding genes are identical, and their distribution seems to be restricted to the species Lactobacillus amylovorus that is of interest for cereal fermentations.", Int. J. Food Microbiol. 90:93-106(2004).
PROTEIN SEQUENCE OF 16-65, AND MASS SPECTROMETRY, STRAIN=LMG P-13139
MEDLINE=97133940;PubMed=8979334;
Contreras B.G.L., De Vuyst L., Devreese B., Busanyova K., Raymaeckers J., Bosman F., Sablon E., Vandamme E.J.
"Isolation, purification, and amino acid sequence of lactobin A, one of the two bacteriocins produced by Lactobacillus amylovorus LMG P- 13139.", Appl. Environ. Microbiol. 63:13-20(1997).
PROTEIN SEQUENCE OF 16-50, STRAIN=DCE 471
PubMed=10517609;
Callewaert R., Holo H., Devreese B., Van Beeumen J., Nes I., De Vuyst L.
"Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method.", Microbiology 145:2559-2568(1999).
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Gene structure
genome of Lactobin-A (Amylovorin-L471)

Gene idNameDescriptionLocation
BACGene388amyLlactobin A bacteriocin16..213
amyL
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 
          |          |          |          |          |          | 
 NRWTNAYSAA LGCAVPGVKY GKKLGGVWGA VIGGVGGAAV CGLAGYVRKG

Wheel representation
wheel representation of Lactobin-A (Amylovorin-L471)
Protein sequence annotations
Features of Lactobin-A (Amylovorin-L471)


FeaturePosition(s)LengthDescription
CHAIN1↔5050
Bacteriocin amylovorin-L.
Feature identifier = PRO_0000002758.
TRANSMEM24↔4421Helical; (Potential).
Composition
Amino acidCountPercent
A816.00 %
C24.00 %
D00.00 %
E00.00 %
F00.00 %
G1326.00 %
H00.00 %
I12.00 %
K48.00 %
L36.00 %
M00.00 %
N24.00 %
P12.00 %
Q00.00 %
R24.00 %
S12.00 %
T12.00 %
V714.00 %
W24.00 %
Y36.00 %
Hydrophobicity

Composition
Formula C220 H348 N64 O58 S2
Absent amino acids DEFHMQ
Common amino acids G
Mass (Da) 4900.57
Net charge +6
Isoelectric point 10.37
Basic residues 6
Acidic residues 0
Hydrophobic residues 21
Polar residues 22
Aliphatic residues 11
Tiny residues 22
Boman Index 10.17
Hydropathy Index 0.38
Aliphatic Index 87.8
Instability Index 10.27 (stable)
Half Life Mammalian : 1.4 hour
Yeast : 3 min
E. coli : >10 hour
Extinction Coefficient 15595 M-1 cm-1
Absorbance 280nm 318.27