Bacteriocin: Brochocin-c
Accession: BAC093
Classification
Class: Unclassified
Genetics
Gene: brcA
Producer and target organisms
Producer Organism: Brochothrix campestris [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesListeriaceaeBrochothrix
Target organismsBacillus - Brochothrix - Carnobacterium - Clostridium - Enterococcus - Lactobacillus - Lactococcus - Leuconostoc - Listeria - Pediococcus - Staphylococcus - Streptococcus
Uniprot and PDB links
UniProt Entry: O85756
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=ATCC43754
MEDLINE=99054910;PubMed=9835559;
McCormick J.K., Poon A., Sailer M., Gao Y., Roy K.L., McMullen L.M., Vederas J.C., Stiles M.E., Van Belkum M.J.
"Genetic characterization and heterologous expression of brochocin-C, an antibotulinal, two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754.", Appl. Environ. Microbiol. 64:4757-4766(1998).
MEDLINE=99054910;PubMed=9835559;
McCormick J.K., Poon A., Sailer M., Gao Y., Roy K.L., McMullen L.M., Vederas J.C., Stiles M.E., Van Belkum M.J.
"Genetic characterization and heterologous expression of brochocin-C, an antibotulinal, two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754.", Appl. Environ. Microbiol. 64:4757-4766(1998).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene380 | transposase ORF1 | 1..399 | |
| BACGene381 | ORF2 | 417..716 | |
| BACGene382 | brcA | bacteriocin BrcA; brochocin-C; antibotulinal | 1273..1506 |
| BACGene383 | brcB | bacteriocin BrcB; brochocin-C; antibotulinal | 1519..1701 |
| BACGene384 | brcI | bacteriocin immunity protein BrcI | 1695..1856 |
| BACGene385 | brcD | transport accessory protein BrcD | 2059..2535 |
| BACGene386 | brcT | ABC translocator BrcT | 2722..4357 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | YSSKDCLKDI GKGIGAGTVA GAAGGGLAAG LGAIPGAFVG AHFGVIGG |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 9 | 18.75 % |
| C | 1 | 2.08 % |
| D | 2 | 4.17 % |
| E | 0 | 0.00 % |
| F | 2 | 4.17 % |
| G | 15 | 31.25 % |
| H | 1 | 2.08 % |
| I | 4 | 8.33 % |
| K | 3 | 6.25 % |
| L | 3 | 6.25 % |
| M | 0 | 0.00 % |
| N | 0 | 0.00 % |
| P | 1 | 2.08 % |
| Q | 0 | 0.00 % |
| R | 0 | 0.00 % |
| S | 2 | 4.17 % |
| T | 1 | 2.08 % |
| V | 3 | 6.25 % |
| W | 0 | 0.00 % |
| Y | 1 | 2.08 % |
Hydrophobicity
Composition
| Formula | C191
H305
N53
O57
S1 |
| Absent amino acids | EMNQRW |
| Common amino acids | G |
| Mass (Da) | 4306.77 |
| Net charge | +2 |
| Isoelectric point | 8.52 |
| Basic residues | 4 |
| Acidic residues | 2 |
| Hydrophobic residues | 21 |
| Polar residues | 20 |
| Aliphatic residues | 10 |
| Tiny residues | 26 |
| Boman Index | 35.93 |
| Hydropathy Index | 0.69 |
| Aliphatic Index | 93.75 |
| Instability Index | 1.54 (stable) |
| Half Life |
Mammalian : 2.8 hour Yeast : 10 min E. coli : 2 min |
| Extinction Coefficient | 1490 M-1 cm-1 |
| Absorbance 280nm | 31.7 |