Carnobacteriocin B2 (Carnocin CP52)

Accession: BAC091

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Bacteriocin: carnobacteriocin B2 (Carnocin CP52)

Accession: BAC091

Classification

Class: class IIA/YGNGV

Genetics

Gene: cbnB2; Synonyms=canCP52

Producer and target organisms

Producer Organism: Carnobacterium piscicola [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesCarnobacteriaceaeCarnobacterium

Target organismsListeria - Enterococcus

Uniprot and PDB links

UniProt Entry: P38580

PDB Entry1CW5 resolved by NMR 1RY3 resolved by NMR

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 19-56, STRAIN=LV17B
MEDLINE=94216339;PubMed=8163526;
Quadri L.E.N., Sailer M., Roy K.L., Vederas J.C., Stiles M.E.
"Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV17B.", J. Biol. Chem. 269:12204-12211(1994).

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=CP5
MEDLINE=98063519;PubMed=9353214;DOI=10.1007/s002849900262
Herbin S., Mathieu F., Brule F., Branlant C., Lefebvre G., Lebrihi A.
"Characteristics and genetic determinants of bacteriocin activities produced by Carnobacterium piscicola CP5 isolated from cheese.", Curr. Microbiol. 35:319-326(1997).

[Ref. 3] | View abstract | Export citation

STRUCTURE BY NMR OF 19-66.
MEDLINE=20039867;PubMed=10569926;DOI=10.1021/bi991351x
Wang Y., Henz M.E., Gallagher N.L.F., Chai S., Gibbs A.C., Yan L.Z., Stiles M.E., Wishart D.S., Vederas J.C.
"Solution structure of carnobacteriocin B2 and implications for structure-activity relationships among type IIa bacteriocins from lactic acid bacteria.", Biochemistry 38:15438-15447(1999).

Gene structure

genome of carnobacteriocin B2 (Carnocin CP52)

Gene id	Name	Description	Location
BACGene356		transposase ORF-1; putative	1..564
BACGene357		transposase ORF-2; putative	complement(491..949)
BACGene358		ORF-3; unknown function	complement(1422..1550)
BACGene359		bacteriocin ORF-4; putative	complement(1776..1922)
BACGene360		bacteriocin ORF-5; putative	complement(1944..2099)
BACGene361		bacteriocin ORF-6; putative	2324..2449
BACGene362	cbnK	histidine protein kinase	2492..3820
BACGene363	cbnR	response regulator	3795..4532
BACGene364	cbnT	ATP-dependent translocator	4558..6708
BACGene365	cbnD	accessory protein	6724..8091
BACGene366	cbnD	ORF-7; unknown function	8849..9178
BACGene367	cbnB2	carnobacteriocin B2	9296..9496
BACGene368	cbiB2	carnobacteriocin B2 immunity protein	9533..9868

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
VNYGNGVSCS KTKCSVNWGQ AFQERYTAGI NSFVSGVASG AGSIGRRP

Wheel representation

3D Structure

View PDB entry: 1CW5

Protein sequence annotations

Features of carnobacteriocin B2 (Carnocin CP52)

Feature	Position(s)	Length	Description
CHAIN	1↔48	48	Bacteriocin carnobacteriocin B2. Feature identifier = PRO_0000002737.
DISULFID	9↔14	6
STRAND	4↔8	5
STRAND	10↔12	3
HELIX	18↔41	24

Composition

Amino acid	Count	Percent
A	4	8.33 %
C	2	4.17 %
D	0	0.00 %
E	1	2.08 %
F	2	4.17 %
G	8	16.67 %
H	0	0.00 %
I	2	4.17 %
K	2	4.17 %
L	0	0.00 %
M	0	0.00 %
N	4	8.33 %
P	1	2.08 %
Q	2	4.17 %
R	3	6.25 %
S	7	14.58 %
T	2	4.17 %
V	5	10.42 %
W	1	2.08 %
Y	2	4.17 %

Hydrophobicity

Composition

Formula	C₂₁₃ H₃₃₂ N₆₆ O₆₈ S₂
Absent amino acids	DHLM
Common amino acids	G
Mass (Da)	4988.27
Net charge	+4
Isoelectric point	9.96
Basic residues	5
Acidic residues	1
Hydrophobic residues	14
Polar residues	25
Aliphatic residues	7
Tiny residues	19
Boman Index	-73.88
Hydropathy Index	-0.28
Aliphatic Index	54.79
Instability Index	18.67 (stable)
Half Life	Mammalian : 100 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	8605 M^-1 cm^-1
Absorbance 280nm	183.09