Enterocin A

Accession: BAC088

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Bacteriocin: Enterocin A

Accession: BAC088
Classification
Class: Class IIa, IIc (problematic)
Genetics
Gene: entA
Producer and target organisms

Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsEnterococcus faecalis JCM 5803 (MIC=64.7nM) - Enterococcus faecalis OU510 (MIC=32.3nM) - Enterococcus faecium JCM 5804 (MIC=200nM) - Enterococcus faecium TUA 1344L (MIC=129nM) - Enterococcus hirae ATCC 10541 (MIC=129nM) - Lactobacillus plantarum ATCC 14917 (MIC=259nM) - Lactobacillus sakei ssp. sakei JCM 1157 (MIC=16.2nM) - Lactococcus lactis ssp. cremoris ATCC 19257 (MIC=4140nM) - Bacillus coagulans JCM 2257 (MIC=8280nM) - Bacillus subtilis JCM 1465 (MIC=4140nM) - Listeria innocua ATCC 33090 (MIC=1040nM) - Enterococcus - Lactobacillus - Pediococcus - Listeria monocytogenes

[Ref. 1] | View abstract | Export citation
NUCLEOTIDE SEQUENCE, STRAIN=CTC492
MEDLINE=96209231;PubMed=8633865;
Aymerich T., Holo H., Havarstein L.S., Hugas M., Garriga M., Nes I.F.
"Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins.", Appl. Environ. Microbiol. 62:1676-1682(1996).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=CTC492
Harvarstein L.
"", Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
[Ref. 3]
NUCLEOTIDE SEQUENCE, STRAIN=N15
Uchiyama K., Losteinkit C., Shioya S.
"Characterization of bacteriocin produced by Enterococcus faecium N15 and cloning of the related genes.", Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
[Ref. 4] | View abstract | Export citation
NUCLEOTIDE SEQUENCE, STRAIN=DPC1146
MEDLINE=99203104;PubMed=10103244;
O'Keeffe T., Hill C., Ross R.P.
"Characterization and heterologous expression of the genes encoding enterocin A production, immunity, and regulation in Enterococcus faecium DPC1146.", Appl. Environ. Microbiol. 65:1506-1515(1999).
[Ref. 5] | View abstract | Export citation
NUCLEOTIDE SEQUENCE, STRAIN=KU-B5.
PubMed=20418437;DOI=10.1128/AEM.02264-09
Hu C.-B., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.", Appl. Environ. Microbiol. 76:4542-4545(2010).
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Gene structure
Unknown error type: [8] A non well formed numeric value encountered
genome of Enterocin A

Gene idNameDescriptionLocation
BACGene319IS1216 transposase21380..21806,1
BACGene320ribonucleoside-diphosphate reductase 2, NrdH-redoxincomplement(284..448)
BACGene321hypothetical protein570..704
BACGene322Fe2+ transport system protein A849..1322
BACGene323Ferrous iron transport protein B1319..3451
BACGene324hypothetical protein3453..3587
BACGene325GntR family transcription regulator3681..4325
BACGene326Glycine betaine ABC transport system, ATP-binding protein OpuAA4512..5705
BACGene327Glycine betaine ABC transport system, permease protein OpuAB / Glycine betaineABC transport system, glycine betaine-binding protein OpuAC5698..7425
BACGene328hypothetical protein7819..8016
BACGene329probable immunity protein8018..8329
BACGene330entFEntF8433..8579
BACGene331entFhypothetical proteincomplement(8576..9529)
BACGene332entFhypothetical proteincomplement(9612..9743)
BACGene333entFResolvase, N-terminal domain10172..10735
BACGene334entFhypothetical proteincomplement(11014..11301)
BACGene335entFhypothetical proteincomplement(11291..11620)
BACGene336entFCadmium efflux system accessory protein11925..12254
BACGene337vanZProtein vanZcomplement(12541..13026)
BACGene338vanZD-alanyl-D-alanine carboxypeptidasecomplement(13179..14090)
BACGene339vanXvancomycin B-type resistance protein VanXcomplement(14518..15126)
BACGene340vanAVancomycin Teicoplanin A-type resistance protein VanA / D-alanine--D-alanineligasecomplement(15132..16163)
BACGene341vanHVancomycin resistance protein VanH / D-lactate dehydrogenasecomplement(16156..17124)
BACGene342vanHTransposase, IS204/IS1001/IS1096/IS116517456..18748
BACGene343vanSSensor histidine kinase VanScomplement(18846..19988)
BACGene344vanSvancomycin response regulatorcomplement(19978..20673)
BACGene345vanSTransposon Tn1546 resolvasecomplement(20887..21318)
entFentFentFentFentFentFentFvanZvanZvanXvanAvanHvanHvanSvanSvanS
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 TTHSGKYYGN GVYCTKNKCT VDWAKATTCI AGMSIGGFLG GAIPGKC
Wheel representation
wheel representation of Enterocin A
Protein sequence annotations
Features of Enterocin A


FeaturePosition(s)LengthDescription
CHAIN1↔4747
NON_TER47
Composition
Amino acidCountPercent
A48.51 %
C48.51 %
D12.13 %
E00.00 %
F12.13 %
G919.15 %
H12.13 %
I36.38 %
K510.64 %
L12.13 %
M12.13 %
N24.26 %
P12.13 %
Q00.00 %
R00.00 %
S24.26 %
T612.77 %
V24.26 %
W12.13 %
Y36.38 %
Hydrophobicity

Composition
Formula C211 H329 N57 O63 S5
Absent amino acids EQR
Common amino acids G
Mass (Da) 4851.38
Net charge +5
Isoelectric point 8.98
Basic residues 6
Acidic residues 1
Hydrophobic residues 12
Polar residues 26
Aliphatic residues 6
Tiny residues 15
Boman Index -20.81
Hydropathy Index -0.03
Aliphatic Index 54.04
Instability Index 25.08 (stable)
Half Life Mammalian : 7.2 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 10220 M-1 cm-1
Absorbance 280nm 222.17