Lactococcin-B

Accession: BAC087

Default view

Bacteriocin: lactococcin-B

Accession: BAC087

Classification

Class: ~~Unclassified~~

Genetics

Gene: lcnB

Producer and target organisms

Producer Organism: Lactococcus lactis subsp (Streptococcus cremoris), cremoris [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeLactococcus

Target organismsLactococci

Description

Mode of action:
Kills Lactococci by dissipating the membrane potential of the cells.

Uniprot and PDB links

UniProt Entry: P35518

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=9B4
MEDLINE=92304065;PubMed=1610182;
van Belkum M.J., Kok J., Venema G.
"Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6.", Appl. Environ. Microbiol. 58:572-577(1992).

[Ref. 2] | View abstract | Export citation

MUTAGENESIS OF CYS-45.
MEDLINE=97039852;PubMed=8885398;
Venema K., Dost M.H., Venema G., Kok J.
"Mutational analysis and chemical modification of Cys24 of lactococcin B, a bacteriocin produced by Lactococcus lactis.", Microbiology 142:2825-2830(1996).

Gene structure

Gene id	Name	Description	Location
BACGene316	lciA	lactococcin A immunity; This sequence comes from Fig. 2	1..75
BACGene317	lcnB	bacteriocin lactococcin B This sequence comes from Fig. 2.	350..556
BACGene318	lciB	lactococcin B immunity; This sequence comes from Fig. 2	583..858

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
SLQYVMSAGP YTWYKDTRTG KTICKQTIDT ASYTFGVMAE GWGKTFH

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔47	47	Bacteriocin lactococcin-B. Feature identifier = PRO_0000002771.
MUTAGEN	24		C->A,D,E,F,G,I,L,M,N,P,Q,S,T,V,Y,W: Noloss of activity.
MUTAGEN	24		C->R,K: Loss of activity.

Composition

Amino acid	Count	Percent
A	3	6.38 %
C	1	2.13 %
D	2	4.26 %
E	1	2.13 %
F	2	4.26 %
G	5	10.64 %
H	1	2.13 %
I	2	4.26 %
K	4	8.51 %
L	1	2.13 %
M	2	4.26 %
N	0	0.00 %
P	1	2.13 %
Q	2	4.26 %
R	1	2.13 %
S	3	6.38 %
T	8	17.02 %
V	2	4.26 %
W	2	4.26 %
Y	4	8.51 %

Hydrophobicity

Composition

Formula	C₂₄₁ H₃₅₈ N₆₀ O₇₁ S₃
Absent amino acids	N
Common amino acids	T
Mass (Da)	5346.8
Net charge	+3
Isoelectric point	9.1
Basic residues	6
Acidic residues	3
Hydrophobic residues	12
Polar residues	21
Aliphatic residues	5
Tiny residues	11
Boman Index	-58.86
Hydropathy Index	-0.44
Aliphatic Index	43.62
Instability Index	19.18 (stable)
Half Life	Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	16960 M^-1 cm^-1
Absorbance 280nm	368.7