Bacteriocin: Coagulin A
Accession: BAC084
Classification
Class: Unclassified
Genetics
Gene: coaA
Producer and target organisms
Producer Organism: Bacillus coagulans [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesBacillaceaeBacillus
Target organismsBacillus coagulans - Enterococcus faecium - Leuconostoc mesenteroides - Listeria innocua - Listeria monocytogenes - Listeria seeligeri - Oenococcus oeni - Pediococcus pentosaceus - Pediococcus damnosus
Uniprot and PDB links
UniProt Entry: Q9EZB2
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=I4
MEDLINE=20551094;PubMed=11097892;DOI=10.1128/AEM.66.12.5213-5220.2000
Le Marrec C., Hyronimus B., Bressollier P., Verneuil B., Urdaci M.C.
"Biochemical and genetic characterization of coagulin, a new antilisterial bacteriocin in the pediocin family of bacteriocins, produced by Bacillus coagulans I(4).", Appl. Environ. Microbiol. 66:5213-5220(2000).
MEDLINE=20551094;PubMed=11097892;DOI=10.1128/AEM.66.12.5213-5220.2000
Le Marrec C., Hyronimus B., Bressollier P., Verneuil B., Urdaci M.C.
"Biochemical and genetic characterization of coagulin, a new antilisterial bacteriocin in the pediocin family of bacteriocins, produced by Bacillus coagulans I(4).", Appl. Environ. Microbiol. 66:5213-5220(2000).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=I4
Le Henaff-Le Marrec C., Hyronimus B., Bressolier P.
"", Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Le Henaff-Le Marrec C., Hyronimus B., Bressolier P.
"", Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene306 | coaA | CoaA pediocin-like bacteriocin; similar to PedA from Pediococcus acidilactici | 1089..1277 |
| BACGene307 | coaB | CoaB immunity protein; similar to PedB from Pediococcus acidilactici | 1315..1653 |
| BACGene308 | coaC | CoaC accessory protein; similar to PedC from Pediococcus acidilactici | 1677..2201 |
| BACGene309 | coaD | CoaD ABC transporter; similar to PedD from Pediococcus acidilactici | 2300..4474 |
| BACGene310 | mob/pre | Mob/Pre putative plasmid recombination enzyme; similar to various Mob/Pre proteins fromgram positive bacteria plasmids | complement(4594..6018) |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | KYYGNGVTCG KHSCSVDWGK ATTCIINNGA MAWATGGHQG THKC |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 4 | 9.09 % |
| C | 4 | 9.09 % |
| D | 1 | 2.27 % |
| E | 0 | 0.00 % |
| F | 0 | 0.00 % |
| G | 8 | 18.18 % |
| H | 3 | 6.82 % |
| I | 2 | 4.55 % |
| K | 4 | 9.09 % |
| L | 0 | 0.00 % |
| M | 1 | 2.27 % |
| N | 3 | 6.82 % |
| P | 0 | 0.00 % |
| Q | 1 | 2.27 % |
| R | 0 | 0.00 % |
| S | 2 | 4.55 % |
| T | 5 | 11.36 % |
| V | 2 | 4.55 % |
| W | 2 | 4.55 % |
| Y | 2 | 4.55 % |
Hydrophobicity
Composition
| Formula | C196
H298
N60
O60
S5 |
| Absent amino acids | EFLPR |
| Common amino acids | G |
| Mass (Da) | 4633.95 |
| Net charge | +6 |
| Isoelectric point | 8.67 |
| Basic residues | 7 |
| Acidic residues | 1 |
| Hydrophobic residues | 10 |
| Polar residues | 24 |
| Aliphatic residues | 4 |
| Tiny residues | 14 |
| Boman Index | -45.48 |
| Hydropathy Index | -0.43 |
| Aliphatic Index | 40 |
| Instability Index | -0.48 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 14230 M-1 cm-1 |
| Absorbance 280nm | 330.93 |