Enterocin P

Accession: BAC079

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Bacteriocin: Enterocin P

Accession: BAC079

Classification

Class: Class IIa, IIb and IIc (problematic)

Genetics

Gene: entP

Producer and target organisms

Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsLactobacillus sakei - Enterococcus faecium

Description

Enterococcus faecium L50 produces, in addition to EntL50, the sec-dependent pediocin-like enterocin P (EntP), and an unmodified non-pediocin-like bacteriocin, termed enterocin Q (EntQ), synthesized without an N-terminal leader sequence or signal peptide. Note=Ref2.

Classification note:
Enterocin P classification is problematic since it classified in class IIa, IIb and IIc according to literature. To review its classification, please see Zouhir et al., 2007.

Uniprot and PDB links

UniProt Entry: O30434

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=L50
MEDLINE=20528333;PubMed=11073927;DOI=10.1128/JB.182.23.6806-6814.2000
Cintas L.M., Casaus P., Herranz C., Havarstein L.S., Holo H., Hernandez P.E., Nes I.F.
"Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q.", J. Bacteriol. 182:6806-6814(2000).

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=P13
MEDLINE=98027370;PubMed=9361419;
Cintas L.M., Casaus P., Havarstein L.S., Hernandez P.E., Nes I.F.
"Biochemical and genetic characterization of enterocin P, a novel sec- dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum.", Appl. Environ. Microbiol. 63:4321-4330(1997).

Gene structure

Gene id	Name	Description	Location
BACGene276	entP	enterocin P precursor broad antimicrobial spectrum pediocin-like bacteriocin secreted by the generalsecretory pathway; sec-dependent bacteriocin	173..388
BACGene277	entP	putative bacteriocin immunity protein orf2	393..659

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
ATRSYGNGVY CNNSKCWVNW GEAKENIAGI VISGWASGLA GMGH

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔44	44	Bacteriocin enterocin-P. Feature identifier = PRO_5000053228.
DISULFID	11↔16	6	By similarity.

Composition

Amino acid	Count	Percent
A	5	11.36 %
C	2	4.55 %
D	0	0.00 %
E	2	4.55 %
F	0	0.00 %
G	8	18.18 %
H	1	2.27 %
I	3	6.82 %
K	2	4.55 %
L	1	2.27 %
M	1	2.27 %
N	5	11.36 %
P	0	0.00 %
Q	0	0.00 %
R	1	2.27 %
S	4	9.09 %
T	1	2.27 %
V	3	6.82 %
W	3	6.82 %
Y	2	4.55 %

Hydrophobicity

Composition

Formula	C₂₀₂ H₃₀₃ N₅₉ O₆₁ S₃
Absent amino acids	DFPQ
Common amino acids	G
Mass (Da)	4648.91
Net charge	+2
Isoelectric point	8.22
Basic residues	4
Acidic residues	2
Hydrophobic residues	15
Polar residues	22
Aliphatic residues	7
Tiny residues	17
Boman Index	-33.68
Hydropathy Index	-0.15
Aliphatic Index	66.59
Instability Index	13.31 (stable)
Half Life	Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	19605 M^-1 cm^-1
Absorbance 280nm	455.93