Sakacin-P (Sakacin 674)

Accession: BAC078

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Bacteriocin: sakacin-P (Sakacin 674)

Accession: BAC078

Classification

Class: class IIA/YGNGV

Genetics

Gene: sakP; Synonyms=sakR, sppA

Producer and target organisms

Producer Organism: Lactobacillus sakei [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesLactobacillaceaeLactobacillus

Target organismsLactobacilli - Carnobacteria - Enterococcus faecalis - Brochothrix thermosphacta - Listeria monocytogenes - Listeria innocua - Listeria ivanovii

Related Entries

sakacin-A Sakacin G

Uniprot and PDB links

UniProt Entry: P35618 Q57121

PDB Entry1OG7 resolved by NMR 1OHM resolved by NMR 1OHN resolved by NMR

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=LTH673
MEDLINE=94236240;PubMed=8180701;
Tichaczek P.S., Vogel R.F., Hammes W.P.
"Cloning and sequencing of sakP encoding sakacin P, the bacteriocin produced by Lactobacillus sake LTH 673.", Microbiology 140:361-367(1994).

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-61, AND CHARACTERIZATION, STRAIN=Lb674
MEDLINE=94186010;PubMed=8138128;DOI=10.1016/0378-1097(94)90005-1
Holck A.L., Axelsson L., Huehne K., Kroeckel L.
"Purification and cloning of sakacin 674, a bacteriocin from Lactobacillus sake Lb674.", FEMS Microbiol. Lett. 115:143-150(1994).

[Ref. 3] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=Lb674
MEDLINE=96262715;PubMed=8704983;
Huehne K., Axelsson L., Holck A., Kroeckel L.
"Analysis of the sakacin P gene cluster from Lactobacillus sake Lb674 and its expression in sakacin-negative Lb. sake strains.", Microbiology 142:1437-1448(1996).

[Ref. 4]

PROTEIN SEQUENCE OF 19-59, AND CHARACTERIZATION, STRAIN=LTH673
Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P.
"Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from L. sake LTH673.", Syst. Appl. Microbiol. 15:460-465(1992).

Gene structure

Gene id	Name	Description	Location
BACGene270	sppK	SppK putative histidine protein kinase	572..1918
BACGene271	sppR	SppR putative response regulator protein ttg start codon	1920..2666
BACGene272	sppA	SppA bacteriocin preprotein	2813..2998
BACGene273	spiA	SpiA putative protein conferring immunity to sakacin P	3092..3388
BACGene274	sppT	SppT putative ATP-dependent translocator protein	3739..5895
BACGene275	sppE	SppE putative accessory protein for sakacin P export	5910..7286

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
KYYGNGVHCG KHSCTVDWGT AIGNIGNNAA ANWATGGNAG WNK

Wheel representation

3D Structure

View PDB entry: 1OG7

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔43	43	Bacteriocin sakacin-P. Feature identifier = PRO_0000002752.
DISULFID	9↔14	6
STRAND	2↔4	3
STRAND	10↔12	3
HELIX	19↔32	14
TURN	35↔41	7

Composition

Amino acid	Count	Percent
A	6	13.95 %
C	2	4.65 %
D	1	2.33 %
E	0	0.00 %
F	0	0.00 %
G	9	20.93 %
H	2	4.65 %
I	2	4.65 %
K	3	6.98 %
L	0	0.00 %
M	0	0.00 %
N	7	16.28 %
P	0	0.00 %
Q	0	0.00 %
R	0	0.00 %
S	1	2.33 %
T	3	6.98 %
V	2	4.65 %
W	3	6.98 %
Y	2	4.65 %

Hydrophobicity

Composition

Formula	C₁₉₂ H₂₈₀ N₆₀ O₅₉ S₂
Absent amino acids	EFLMPQR
Common amino acids	G
Mass (Da)	4455.57
Net charge	+4
Isoelectric point	8.77
Basic residues	5
Acidic residues	1
Hydrophobic residues	13
Polar residues	24
Aliphatic residues	4
Tiny residues	16
Boman Index	-45.77
Hydropathy Index	-0.57
Aliphatic Index	45.58
Instability Index	21.03 (stable)
Half Life	Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min
Extinction Coefficient	19605 M^-1 cm^-1
Absorbance 280nm	466.79