Bacteriocin: Bacteriocin 31
Accession: BAC076
Classification
Class: Unclassified
Genetics
Gene: bacA
Producer and target organisms
Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsEnterococcus hirae - Enterococcus faecium - Listeria monocytogenes
Uniprot and PDB links
UniProt Entry: Q47778
PDB EntryUnknown
NUCLEOTIDE SEQUENCE.
MEDLINE=96256614;PubMed=8655558;
Tomita H., Fujimoto S., Tanimoto K., Ike Y.
"Cloning and genetic organization of the bacteriocin 31 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pYI17.", J. Bacteriol. 178:3585-3593(1996).
MEDLINE=96256614;PubMed=8655558;
Tomita H., Fujimoto S., Tanimoto K., Ike Y.
"Cloning and genetic organization of the bacteriocin 31 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pYI17.", J. Bacteriol. 178:3585-3593(1996).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene252 | ORF4 | 551..832 | |
| BACGene253 | ORF5 | 849..1133 | |
| BACGene254 | ORF6 | 1135..1368 | |
| BACGene255 | ORF7 | complement(1528..1782) | |
| BACGene256 | ORF8 | complement(1899..2261) | |
| BACGene257 | ORF9 | complement(2324..2566) | |
| BACGene258 | ORF10 | complement(2602..2919) | |
| BACGene259 | ORF11 | complement(3757..4005) | |
| BACGene260 | bacA | 4257..4460 | |
| BACGene261 | bacB | 4477..4761 | |
| BACGene262 | bacB | ORF3 | 4786..5058 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | ATYYGNGLYC NKQKCWVDWN KASREIGKII VNGNVQHGPW APR |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔43 | 43 | Potential. Feature identifier = PRO_5000140060. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 3 | 6.98 % |
| C | 2 | 4.65 % |
| D | 1 | 2.33 % |
| E | 1 | 2.33 % |
| F | 0 | 0.00 % |
| G | 5 | 11.63 % |
| H | 1 | 2.33 % |
| I | 3 | 6.98 % |
| K | 4 | 9.30 % |
| L | 1 | 2.33 % |
| M | 0 | 0.00 % |
| N | 5 | 11.63 % |
| P | 2 | 4.65 % |
| Q | 2 | 4.65 % |
| R | 2 | 4.65 % |
| S | 1 | 2.33 % |
| T | 1 | 2.33 % |
| V | 3 | 6.98 % |
| W | 3 | 6.98 % |
| Y | 3 | 6.98 % |
Hydrophobicity
Composition
| Formula | C222
H333
N65
O60
S2 |
| Absent amino acids | FM |
| Common amino acids | GN |
| Mass (Da) | 4955.33 |
| Net charge | +5 |
| Isoelectric point | 9.72 |
| Basic residues | 7 |
| Acidic residues | 2 |
| Hydrophobic residues | 13 |
| Polar residues | 17 |
| Aliphatic residues | 7 |
| Tiny residues | 9 |
| Boman Index | -71.42 |
| Hydropathy Index | -0.75 |
| Aliphatic Index | 63.49 |
| Instability Index | 18.33 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 21095 M-1 cm-1 |
| Absorbance 280nm | 502.26 |