Bacteriocin: thermophilin 13
Accession: BAC074
Classification
Class: Unclassified
Genetics
Gene: thermophilin 13 thmB
Producer and target organisms
Producer Organism: Streptococcus thermophilus [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Target organismsEnterococcus - Lactobacillus - Lactococcus - Leuconostoc
Uniprot and PDB links
UniProt Entry: O54455
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=SFi13
MEDLINE=97306337;PubMed=9162062;DOI=10.1074/jbc.272.22.14277
Marciset O., Jeronimus-Stratingh M.C., Mollet B., Poolman B.
"Thermophilin 13, a nontypical antilisterial poration complex bacteriocin, that functions without a receptor.", J. Biol. Chem. 272:14277-14284(1997).
MEDLINE=97306337;PubMed=9162062;DOI=10.1074/jbc.272.22.14277
Marciset O., Jeronimus-Stratingh M.C., Mollet B., Poolman B.
"Thermophilin 13, a nontypical antilisterial poration complex bacteriocin, that functions without a receptor.", J. Biol. Chem. 272:14277-14284(1997).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene249 | thmA | amphipathic pore-forming peptide precursor ThmA | 79..336 |
| BACGene250 | thmB | enhancer peptide for thermophilin 13 precursor requires ThmB for full activity | 353..547 |
| BACGene251 | thermophilin 13 operon | unknown hydrophobic peptide | 537..695 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | QINWGSVVGH CIGGAIIGGA FSGGAAAGVG CLVGSGKAII NGL |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔43 | 43 | enhancer peptide for thermophilin 13. Feature identifier = PRO_5000145346. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 6 | 13.95 % |
| C | 2 | 4.65 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 1 | 2.33 % |
| G | 13 | 30.23 % |
| H | 1 | 2.33 % |
| I | 6 | 13.95 % |
| K | 1 | 2.33 % |
| L | 2 | 4.65 % |
| M | 0 | 0.00 % |
| N | 2 | 4.65 % |
| P | 0 | 0.00 % |
| Q | 1 | 2.33 % |
| R | 0 | 0.00 % |
| S | 3 | 6.98 % |
| T | 0 | 0.00 % |
| V | 4 | 9.30 % |
| W | 1 | 2.33 % |
| Y | 0 | 0.00 % |
Hydrophobicity
Composition
| Formula | C172
H278
N50
O50
S2 |
| Absent amino acids | DEMPRTY |
| Common amino acids | G |
| Mass (Da) | 3929.3 |
| Net charge | +2 |
| Isoelectric point | 8.24 |
| Basic residues | 2 |
| Acidic residues | 0 |
| Hydrophobic residues | 20 |
| Polar residues | 20 |
| Aliphatic residues | 12 |
| Tiny residues | 22 |
| Boman Index | 47.24 |
| Hydropathy Index | 1.02 |
| Aliphatic Index | 113.49 |
| Instability Index | 0.72 (stable) |
| Half Life |
Mammalian : 0.8 hour Yeast : 10 min E. coli : >10 hour |
| Extinction Coefficient | 5625 M-1 cm-1 |
| Absorbance 280nm | 133.93 |