Bacteriocin: leucocin C
Accession: BAC071
Classification
Class: class IIA/YGNGV
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Leuconostoc mesenteroides [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLeuconostoc
Target organismsUnknown error type: [8] Uninitialized string offset: -1
lactic acid bacteria (LAB).
Description
Leuconostoc mesenteroides TA33a produces three bacteriocins leucocins A-, B- and C. A partial sequence of 36 amino acids of leucocin C-TA33a (4598 Da) was determined.Leucocin C-TA33a belongs to the class II bacteriocins having the consensus YGNGV motif. Circular dichroism spectra of the leucocins in aqueous solution and micellar SDS indicated that they undergo a structural transition when in a membrane-mimicking environment. Theoretical predictions from circular dichroism data suggest that leucocin C adopt a beta-structure (48%) in membrane-mimicking environments.
Uniprot and PDB links
UniProt Entry: P81053
PDB EntryUnknown
PROTEIN SEQUENCE, STRAIN=TA33a
MEDLINE=98274743;PubMed=9611809;
Papathanasopoulos M.A., Dykes G.A., Revol-Junelles A.-M., Delfour A., von Holy A., Hastings J.W.
"Sequence and structural relationships of leucocins A-, B- and C-TA33a from Leuconostoc mesenteroides TA33a.", Microbiology 144:1343-1348(1998).
MEDLINE=98274743;PubMed=9611809;
Papathanasopoulos M.A., Dykes G.A., Revol-Junelles A.-M., Delfour A., von Holy A., Hastings J.W.
"Sequence and structural relationships of leucocins A-, B- and C-TA33a from Leuconostoc mesenteroides TA33a.", Microbiology 144:1343-1348(1998).
PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
MEDLINE=22122295;PubMed=12127968;DOI=10.1016/S0006-291X(02)00769-6
Fimland G., Sletten K., Nissen-Meyer J.
"The complete amino acid sequence of the pediocin-like antimicrobial peptide leucocin C.", Biochem. Biophys. Res. Commun. 295:826-827(2002).
MEDLINE=22122295;PubMed=12127968;DOI=10.1016/S0006-291X(02)00769-6
Fimland G., Sletten K., Nissen-Meyer J.
"The complete amino acid sequence of the pediocin-like antimicrobial peptide leucocin C.", Biochem. Biophys. Res. Commun. 295:826-827(2002).
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Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | KNYGNGVHCT KKGCSVDWGY AWTNIANNSV MNGLTGGNAG WHN |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔43 | 43 | Bacteriocin leucocin-C. Feature identifier = PRO_0000110575. |
| DISULFID | 9↔14 | 6 | By similarity. |
| CONFLICT | 22 | W -> A (in Ref. 2; AA sequence). |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 3 | 6.98 % |
| C | 2 | 4.65 % |
| D | 1 | 2.33 % |
| E | 0 | 0.00 % |
| F | 0 | 0.00 % |
| G | 8 | 18.60 % |
| H | 2 | 4.65 % |
| I | 1 | 2.33 % |
| K | 3 | 6.98 % |
| L | 1 | 2.33 % |
| M | 1 | 2.33 % |
| N | 8 | 18.60 % |
| P | 0 | 0.00 % |
| Q | 0 | 0.00 % |
| R | 0 | 0.00 % |
| S | 2 | 4.65 % |
| T | 3 | 6.98 % |
| V | 3 | 6.98 % |
| W | 3 | 6.98 % |
| Y | 2 | 4.65 % |
Hydrophobicity
Composition
| Formula | C198
H291
N61
O61
S3 |
| Absent amino acids | EFPQR |
| Common amino acids | GN |
| Mass (Da) | 4616.77 |
| Net charge | +4 |
| Isoelectric point | 8.77 |
| Basic residues | 5 |
| Acidic residues | 1 |
| Hydrophobic residues | 11 |
| Polar residues | 25 |
| Aliphatic residues | 5 |
| Tiny residues | 13 |
| Boman Index | -55.79 |
| Hydropathy Index | -0.67 |
| Aliphatic Index | 45.35 |
| Instability Index | 18.09 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 19605 M-1 cm-1 |
| Absorbance 280nm | 466.79 |