Divergicin M35

Accession: BAC070

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Bacteriocin: Divergicin M35

Accession: BAC070

Classification

Class: class IIa/YGNGV

Genetics

Gene: ~~Unidentified~~

Producer and target organisms

Producer Organism: Carnobacterium divergens (Lactobacillus divergens) [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesCarnobacteriaceaeCarnobacterium

Target organismsListeria monocytogenes - Listeria seeligeri - Listeria welshimeri - Listeria grayi - Listeria murrayi - Listeria ivanovii - Listeria innocua - Carnobacterium divergens - Carnobacterium piscicola -

No antibacterial activity against Lactobacillus lactis - Pediococcus acidilactici - Pediococcus pentosaceus - Lactobacillus salivarius - Lactobacillus delbrueckii - Lactobacillus acidophilus - Lactobacillus plantarum - Lactobacillus casei - Lactobacillus rhamnosus - Streptomyces thermophilus - Propionibacterium freudenreichii - Bifidobacterium breve - Escherichia coli

Description

Has potential for use as a biopreservative for inhibiting Listeria monocytogenes in seafood products.

Related Entries

Divergicin 750 Divergicin A

Uniprot and PDB links

UniProt Entry: P84962

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

PROTEIN SEQUENCE, STRAIN=M35
PubMed=15541799;
Tahiri I, Desbiens M, Benech R, Kheadr E, Lacroix C, Thibault S, Ouellet D, Fliss I.
"Purification, characterization and amino acid sequencing of divergicin M35: a novel class IIa bacteriocin produced by Carnobacterium divergens M35.", Int J Food Microbiol. 97(2):123-36(2004).

[Ref. 2] | View abstract | Export citation

MODE OF ACTION, STRAIN=M35
PubMed=16405682;
Naghmouchi K, Drider D, Kheadr E, Lacroix C, Prevost H, Fliss I.
"Multiple characterizations of Listeria monocytogenes sensitive and insensitive variants to divergicin M35, a new pediocin-like bacteriocin.", J Appl Microbiol. 2006;100(1):29-39.

[Ref. 3] | View abstract | Export citation

MODE OF ACTION, STRAIN=M35
PubMed=17578415;DOI=10.1111/j.1365-2672.2006.03206.x
Naghmouchi K., Drider D., Fliss I.
"Action of divergicin M35, a class IIa bacteriocin, on liposomes and Listeria.", Journal of Applied Microbiology 102(6):1508-17

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
TKYYGNGVYC NSKKCWVDWG TAQGCIDVVI GQLGGGIPGK GKC

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
PEPTIDE	1↔43	43	Bacteriocin divergicin M35. Feature identifier = PRO_0000250606.

Composition

Amino acid	Count	Percent
A	1	2.33 %
C	4	9.30 %
D	2	4.65 %
E	0	0.00 %
F	0	0.00 %
G	10	23.26 %
H	0	0.00 %
I	3	6.98 %
K	5	11.63 %
L	1	2.33 %
M	0	0.00 %
N	2	4.65 %
P	1	2.33 %
Q	2	4.65 %
R	0	0.00 %
S	1	2.33 %
T	2	4.65 %
V	4	9.30 %
W	2	4.65 %
Y	3	6.98 %

Hydrophobicity

Composition

Formula	C₂₀₀ H₃₀₈ N₅₄ O₅₈ S₄
Absent amino acids	EFHMR
Common amino acids	G
Mass (Da)	4543.96
Net charge	+3
Isoelectric point	8.65
Basic residues	5
Acidic residues	2
Hydrophobic residues	11
Polar residues	22
Aliphatic residues	8
Tiny residues	12
Boman Index	-21.68
Hydropathy Index	-0.19
Aliphatic Index	65.58
Instability Index	-3.74 (stable)
Half Life	Mammalian : 7.2 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	15720 M^-1 cm^-1
Absorbance 280nm	374.29