Bacteriocin: carnobacteriocin BM1 (Carnobacteriocin B1)
Accession: BAC069
Classification
Class: class IIA/YGNGV
Genetics
Gene: cbnBM1
Producer and target organisms
Producer Organism: Carnobacterium piscicola [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesCarnobacteriaceaeCarnobacterium
Target organismsListeria - Enterococcus
Uniprot and PDB links
UniProt Entry: P38579
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 19-56, STRAIN=LV17B
MEDLINE=94216339;PubMed=8163526;
Quadri L.E.N., Sailer M., Roy K.L., Vederas J.C., Stiles M.E.
"Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV17B.", J. Biol. Chem. 269:12204-12211(1994).
MEDLINE=94216339;PubMed=8163526;
Quadri L.E.N., Sailer M., Roy K.L., Vederas J.C., Stiles M.E.
"Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV17B.", J. Biol. Chem. 269:12204-12211(1994).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene242 | carnobacteriocin BM1 putative | 103..288 | |
| BACGene243 | carnobacteriocin BM1 immunity protein putative; 'Putative Immunity protein of Carnobacteriocin BM1' | 303..569 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | AISYGNGVYC NKEKCWVNKA ENKQAITGIV IGGWASSLAG MGH |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔43 | 43 | Bacteriocin carnobacteriocin BM1. Feature identifier = PRO_0000002735. |
| DISULFID | 10↔15 | 6 | Probable. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 5 | 11.63 % |
| C | 2 | 4.65 % |
| D | 0 | 0.00 % |
| E | 2 | 4.65 % |
| F | 0 | 0.00 % |
| G | 7 | 16.28 % |
| H | 1 | 2.33 % |
| I | 4 | 9.30 % |
| K | 4 | 9.30 % |
| L | 1 | 2.33 % |
| M | 1 | 2.33 % |
| N | 4 | 9.30 % |
| P | 0 | 0.00 % |
| Q | 1 | 2.33 % |
| R | 0 | 0.00 % |
| S | 3 | 6.98 % |
| T | 1 | 2.33 % |
| V | 3 | 6.98 % |
| W | 2 | 4.65 % |
| Y | 2 | 4.65 % |
Hydrophobicity
Composition
| Formula | C199
H310
N56
O59
S3 |
| Absent amino acids | DFPR |
| Common amino acids | G |
| Mass (Da) | 4545.91 |
| Net charge | +3 |
| Isoelectric point | 8.76 |
| Basic residues | 5 |
| Acidic residues | 2 |
| Hydrophobic residues | 15 |
| Polar residues | 19 |
| Aliphatic residues | 8 |
| Tiny residues | 15 |
| Boman Index | -23.71 |
| Hydropathy Index | -0.08 |
| Aliphatic Index | 77.21 |
| Instability Index | 26.93 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 335.83 |