Thermophilin A

Accession: BAC068

Default view

Bacteriocin: thermophilin A

Accession: BAC068

Classification

Class: ~~Unclassified~~

Genetics

Gene: thermophilin A

Producer and target organisms

Producer Organism: Streptococcus thermophilus [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsStreptococcus - Enterococcus - Lactococcus - Lactobacillus - Leuconostoc - Bifidobacterium - Listeria innocua - Listeria monocytogenes - Bacillus - Clostridium - Staphylococcus

Related Entries

thermophilin 13 Thermophilin 1277

Uniprot and PDB links

UniProt Entry: O54454

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=SFi13
MEDLINE=97306337;PubMed=9162062;DOI=10.1074/jbc.272.22.14277
Marciset O., Jeronimus-Stratingh M.C., Mollet B., Poolman B.
"Thermophilin 13, a nontypical antilisterial poration complex bacteriocin, that functions without a receptor.", J. Biol. Chem. 272:14277-14284(1997).

Gene structure

Gene id	Name	Description	Location
BACGene239	thmA	amphipathic pore-forming peptide precursor ThmA	79..336
BACGene240	thmB	enhancer peptide for thermophilin 13 precursor requires ThmB for full activity	353..547
BACGene241	thermophilin 13 operon	unknown hydrophobic peptide	537..695

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
GALWGAPAGG VGALPGAFVG AHVGAIAGGF ACMGGMIGNK FN

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔42	42	amphipathic pore-forming peptide. Feature identifier = PRO_5000145345.

Composition

Amino acid	Count	Percent
A	9	21.43 %
C	1	2.38 %
D	0	0.00 %
E	0	0.00 %
F	3	7.14 %
G	13	30.95 %
H	1	2.38 %
I	2	4.76 %
K	1	2.38 %
L	2	4.76 %
M	2	4.76 %
N	2	4.76 %
P	2	4.76 %
Q	0	0.00 %
R	0	0.00 %
S	0	0.00 %
T	0	0.00 %
V	3	7.14 %
W	1	2.38 %
Y	0	0.00 %

Hydrophobicity

Composition

Formula	C₁₇₃ H₂₆₂ N₄₈ O₄₅ S₃
Absent amino acids	DEQRSTY
Common amino acids	G
Mass (Da)	3849.21
Net charge	+2
Isoelectric point	8.55
Basic residues	2
Acidic residues	0
Hydrophobic residues	20
Polar residues	16
Aliphatic residues	7
Tiny residues	22
Boman Index	54.07
Hydropathy Index	0.87
Aliphatic Index	79.29
Instability Index	37.13 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	5500 M^-1 cm^-1
Absorbance 280nm	134.15