Bacteriocin: sakacin-A
Accession: BAC066
Classification
Class: class IIA/YGNGV
Genetics
Gene: sapA; Synonyms=sakA
Producer and target organisms
Producer Organism: Lactobacillus sakei [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsinhibits closely related Lactobacilli - Enterococci - Listeria monocytogenes - Listeria ivanovii - Enterococcus faecalis - Carnobacterium sp - Brochothrix thermosphacta
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE, STRAIN=Lb706
MEDLINE=93139766;PubMed=1487735;
Holck A.L., Axelsson L., Birkeland S.-E., Aukrust T., Blom H.
"Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Gen. Microbiol. 138:2715-2720(1992).
MEDLINE=93139766;PubMed=1487735;
Holck A.L., Axelsson L., Birkeland S.-E., Aukrust T., Blom H.
"Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Gen. Microbiol. 138:2715-2720(1992).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=Lb706
MEDLINE=95238285;PubMed=7721704;
Axelsson L., Holck A.
"The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Bacteriol. 177:2125-2137(1995).
MEDLINE=95238285;PubMed=7721704;
Axelsson L., Holck A.
"The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Bacteriol. 177:2125-2137(1995).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene231 | orf1 | complement(5..268) | |
| BACGene232 | saiA | sakacin A immunity factor start codon ttg | complement(653..925) |
| BACGene233 | sapA | sakacin A precursor previously designated sakA | complement(940..1119) |
| BACGene234 | orf4 | 2526..2663 | |
| BACGene235 | sapK | histidine kinase putative; previously designated sakB | 2724..4010 |
| BACGene236 | sapR | response regulator putative | 4026..4769 |
| BACGene237 | sapT | ABC exporter putative | 4842..7001 |
| BACGene238 | sapE | accessory factor for ABC exporter putative | 7020..8405 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | ARSYGNGVYC NNKKCWVNRG EATQSIIGGM ISGWASGLAG M |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔41 | 41 | Bacteriocin sakacin-A. Feature identifier = PRO_0000002750. |
| DISULFID | 10↔15 | 6 | By similarity. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 4 | 9.76 % |
| C | 2 | 4.88 % |
| D | 0 | 0.00 % |
| E | 1 | 2.44 % |
| F | 0 | 0.00 % |
| G | 8 | 19.51 % |
| H | 0 | 0.00 % |
| I | 3 | 7.32 % |
| K | 2 | 4.88 % |
| L | 1 | 2.44 % |
| M | 2 | 4.88 % |
| N | 4 | 9.76 % |
| P | 0 | 0.00 % |
| Q | 1 | 2.44 % |
| R | 2 | 4.88 % |
| S | 4 | 9.76 % |
| T | 1 | 2.44 % |
| V | 2 | 4.88 % |
| W | 2 | 4.88 % |
| Y | 2 | 4.88 % |
Hydrophobicity
Composition
| Formula | C184
H288
N56
O56
S4 |
| Absent amino acids | DFHP |
| Common amino acids | G |
| Mass (Da) | 4327.58 |
| Net charge | +3 |
| Isoelectric point | 9.37 |
| Basic residues | 4 |
| Acidic residues | 1 |
| Hydrophobic residues | 12 |
| Polar residues | 21 |
| Aliphatic residues | 6 |
| Tiny residues | 16 |
| Boman Index | -41.86 |
| Hydropathy Index | -0.19 |
| Aliphatic Index | 61.95 |
| Instability Index | 27.63 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 352.63 |